{"product_id":"biolegend-137608","title":"Biolegend, 137608, APC anti-mouse CD335 (NKp46) Antibody, 100μg","description":"\u003cp\u003eCD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: NKP46-IgG1 Fc fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ) Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ) Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed\u003cbr\u003e\nProduct Citations: Glasner A,et al. 2017. Sci Rep.. 10.1038\/s41598-017-12998-w. PubMed Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed Xu R 2023. Cell Reports. 42(2):112054. PubMed Chun J, et al. 2023. Cancers (Basel). 15: . PubMed Miller CM, et al. 2020. J Virol. 94:00:00. PubMed Fan L, et al. 2021. Cancers (Basel). 13:. PubMed Yilmaz B, et al. 2021. Cell Host Microbe. 29(4):650-663.e9. PubMed Forte E, et al. 2020. J Cell Mol Med. . PubMed Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed Iannello A, et al. 2013. J Exp Med. 210:2057. PubMed Huang B, et al. 2015. PLoS One. 10: 0134715. PubMed Parhi L, et al. 2020. Nat Commun. 2.721527778. PubMed Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed Sibilio A, et al. 2022. iScience. 25:103790. PubMed Hu M, et al. 2020. Cancer Immunol Res. 8:1150. PubMed Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed Yue W, et al. 2019. Nat Commun. 10:2025. PubMed Yao C, et al. 2018. Autophagy. 1.854861111. PubMed Baldwin LA, et al. 2022. Nat Commun. 13:6539. PubMed Muchenditsi A, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 313:G39. PubMed Sánchez-del-Campo L, et al. 2021. J Exp Clin Cancer Res. 40:117. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Renner K, et al. 2020. Cell Reports. 29(1):135-150.e9.. PubMed Pisano F, et al. 2014. PLoS One. 9:103541. PubMed Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed Ghosh M, et al. 2021. Cancer Cell. 39(4):494-508.e5. PubMed Kennedy J, et al. 2014. J Exp Med. 211:2519. PubMed\u003cbr\u003e\nRRID: AB_10612749 (BioLegend Cat. No. 137607) AB_10612758 (BioLegend Cat. No. 137608)\u003cbr\u003e\nStructure: Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains\u003cbr\u003e\nDistribution: Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells\u003cbr\u003e\nFunction: NK cells activation\u003cbr\u003e\nLigand\/Receptor: Viral hemagglutinins, heparan sulfate proteoglycans\u003cbr\u003e\nCell Type: NK cells, NKT cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60. 2. Caligiuri MA. 2008. Blood 112:461. 3. Colonna M. 2009. Immunity 31:15.\u003cbr\u003e\nGene ID: 17086\u003cbr\u003e\nUniProt: View information about CD335 on UniProt.org\u003cbr\u003e\nClone: 29A1.4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: NKp46, NCR1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866544230569,"sku":"137608","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-137608","provider":"Iright","version":"1.0","type":"link"}