{"product_id":"biolegend-141306","title":"Biolegend, 141306, PE anti-mouse LAP (TGF-β1) Antibody, 100μg","description":"\u003cp\u003eTransforming growth factor beta (TGF-β) is a cytokine that has critical functions in immune response by regulating Treg and Th17 cells. TGF-β is first synthesized as pro-TGF-β and then it is cleaved by furin proprotein convertase in the Golgi apparatus to produce the dimeric propeptides called latency-associated peptide (LAP) that non-covalently associates with the dimeric mature TGF-β to prevent its activity. This complex can further associate with latent-TGF-β-binding protein (LTBP) to produce a large latent form for deposition onto the extracellular matrix. The latent-TGF-β can be expressed on the membrane of activated Treg cells, immature dendritic cells, megakaryocytes, and platelets.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Mouse Tgfb1-transduced P3U1 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone TW7-20B9 has been reported to not cross-react with human LAP.2 Several anti-LAP antibody clones have been compared and characterized for their LAP reactivity.2 This antibody recognizes recombinant LAP, latent TGF-ß, and pro-TGF-ß. Additional reported applications (for relevant formats) include: Western blotting1, immunoprecipitation1, and neutralization2. TW7-20B9 is able to neutralize certain unconventional T cell-derived forms of TGF-ß activity, but not the distinct 25 kDa free form of TGF-ß.2\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Oida T, et al. 2010. PLoS ONE (FC, IP, WB) Oida T, et al. 2011. PLoS ONE 6:e18365. (Neut) Tu Z, et al. 2012. Invest Ophthalmol Vis Sci. 53:959. PubMed\u003cbr\u003e\nProduct Citations: Saxena V, et al. 2022. Cell Rep. 39:110727. PubMed Araujo Furlan CL, et al. 2018. Front Immunol. 2.149305556. PubMed\u003cbr\u003e\nRRID: AB_10717505 (BioLegend Cat. No. 141305) AB_10720866 (BioLegend Cat. No. 141306)\u003cbr\u003e\nStructure: Dimmers of latency-associated peptide non-covalently associated with dimmers of mature TGF-β\u003cbr\u003e\nDistribution: Many cell types, highly expressed on activated Tregs and platelets\u003cbr\u003e\nFunction: TGF-β controls cell differentiation, tissue morphogenesis, cell growth, inflammation, matrix synthesis, apoptosis, and regulates immune response.\u003cbr\u003e\nLigand\/Receptor: TGF-β receptors\u003cbr\u003e\nCell Type: Dendritic cells, Platelets, Tregs\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines, Growth Factors\u003cbr\u003e\nAntigen References: 1. Oida T, et al. 2010. PLoS 2. Tran D, et al. 2009. P. Natl. Acad. Sci. USA 106:13445. 3. Ochi H, et al. 2006. Nat. Med. 12:627. 4. Oida T, et al. 2003. J. Immunol. 170:2516. 5. Nakamura K. 2001. J. Exp. Med. 194:629. 6. Miyazono K, et al. 1993. Growth Factors 8:11.\u003cbr\u003e\nGene ID: 21803\u003cbr\u003e\nUniProt: View information about LAP on UniProt.org\u003cbr\u003e\nClone: TW7-20B9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Transforming growth factor-beta 1 (TGF-b1), Latency Associated Peptide (LAP), TGFB1, DPD1, TGF-beta1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864057204905,"sku":"141306","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-141306","provider":"Iright","version":"1.0","type":"link"}