{"product_id":"biolegend-141710","title":"Biolegend, 141710, Alexa Fluor® 488 anti-mouse CD206 (MMR) Antibody, 100μg","description":"\u003cp\u003eCD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant mouse CD206 (MMR)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested FC, IHC-F - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed Yang X, et al. 2015. PNAS. 112:2900. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Divakaruni AS et al. 2018. Cell metabolism. 28(3):490-503 . PubMed Arora H, et al. 2019. Immunity. 50:418. PubMed Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed Mitsui K, et al. 2023. J Neuroinflammation. 20:102. PubMed Chen G, et al. 2021. J Mol Cell Cardiol. 159:62. PubMed Zhang Z, et al. 2022. Mol Nutr Food Res. 66:e2200300. PubMed Wang L, et al. 2022. Cell Rep. 41:111462. PubMed Liang Z, et al. 2023. Front Immunol. 13:944286. PubMed Orillion A, et al. 2018. Clin Cancer Res. 24:6383. PubMed Weiss–Sadan T, et al. 2019. Cell Physiol Biochem. 53:550. PubMed Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed Coppo M, et al. 2016. Nat Commun. 7:12254. PubMed Elbaz M, et al. 2015. Mol Oncol. 9: 906-919. PubMed Nasser MW, et al. 2015. Cancer Res . 75: 974-985. PubMed AR P, et al. 2016. Circ Res. 118: 400-409. PubMed Xu DQ, et al. 2021. Acta Pharmacol Sin. 42:1080. PubMed Xiong A, et al. 2022. EBioMedicine. 83:104239. PubMed Song L, et al. 2020. Am J Physiol Endocrinol Metab. 318:E1004. PubMed Hu D, et al. 2020. Am J Physiol Heart Circ Physiol. 318:H1525. PubMed Palathingal Bava E, et al. 2022. JCI Insight. 7:. PubMed Wu H, et al. 2019. Autophagy. :1. PubMed Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed Jiang W, et al. 2021. J Cell Physiol. 236:7711. PubMed Lynch T, et al. 2017. J Mol Cell Cardiol. 102:83-93. PubMed Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed Fields L, et al. 2021. Mol Ther. 29:2554. PubMed Yang X, et al. 2015. Proc Natl Acad Sci U S A. 112:2900. PubMed Luck H, et al. 2019. Nat Commun. 10:3650. PubMed Song J, et al. 2021. Am J Physiol Heart Circ Physiol. 320:H323. PubMed Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed Ahmad F, et al. 2021. J Am Heart Assoc. 10:e017524. PubMed Tu W, et al. 2021. Cell Death Dis. 12:882. PubMed Lee SH, et al. 2022. Nat Commun. 13:5461. PubMed Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed Babazadeh S, et al. 2021. Cell Mol Biol Lett. 26:30. PubMed Molgora M, et al. 2020. Cell. 182:886. PubMed Zhang F, et al. 2021. J Immunol Res. 2021:5521051. PubMed Tang PC, et al. 2022. Adv Sci (Weinh). 9:e2101235. PubMed Barnette DN, et al. 2018. JCI Insight. 3. PubMed Muñoz M, et al. 2022. ACS Nano. . PubMed Xiao M, et al. 2022. Mol Oncol. 16:1026. PubMed Yang F, et al. 2021. Nat Commun. 12:3424. PubMed\u003cbr\u003e\nRRID: AB_10933252 (BioLegend Cat. No. 141709) AB_10900445 (BioLegend Cat. No. 141710)\u003cbr\u003e\nStructure: Type I transmembrane protein, 175 kD, C-type lectin superfamily\u003cbr\u003e\nDistribution: Macrophages, dendritic cells, Langerhans cells, liver endothelial cells\u003cbr\u003e\nFunction: Pathogen recognition, endocytosis and phagocytosis, antigen presentation\u003cbr\u003e\nLigand\/Receptor: Antigen containing mannose, fucose, or an N-acetyl glucosamine\u003cbr\u003e\nCell Type: Dendritic cells, Endothelial cells, Langerhans cells, Macrophages\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501. 2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469. 3. Burgdorf S, et al. 2006. J. Immunol. 176:6770. 4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.\u003cbr\u003e\nGene ID: 17533\u003cbr\u003e\nUniProt: View information about CD206 on UniProt.org\u003cbr\u003e\nClone: C068C2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MMR (macrophage mannose receptor), MR (mannose receptor), MRC1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: Why is mouse CD206 stained intracellularly and not via surface staining?\u003cbr\u003e\nA: Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866085642409,"sku":"141710","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-141710","provider":"Iright","version":"1.0","type":"link"}