{"product_id":"biolegend-141731","title":"Biolegend, 141731, PE\/Dazzle™ 594 anti-mouse CD206 (MMR) Antibody, 25μg","description":"\u003cp\u003eCD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant mouse CD206 (MMR)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Dazzle™ 594 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality testedFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE\/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed Yang X, et al. 2015. PNAS. 112:2900. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed Martins Nascentes Melo L, et al. 2023. J Immunother Cancer. 11:. PubMed Liang Y, et al. 2022. Theranostics. 12:7729. PubMed Denis M, et al. 2023. Front Immunol. 13:1011943. PubMed Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed Zhang F, et al. 2019. Nat Commun. 10:3974. PubMed Wang B, et al. 2022. Nat Commun. 13:3821. PubMed Paterson N, et al. 2022. Elife. 11:. PubMed Kim S, et al. 2022. Redox Biol. 54:102347. PubMed Dangaj D, et al. 2019. Cancer Cell. 35:885. PubMed Cunha LD et al. 2018. Cell. 175(2):429-441 . PubMed Ahrends T, et al. 2022. STAR Protoc. 3:101157. PubMed Silva HM, et al. 2019. J Exp Med. 216:786. PubMed Zhang L, et al. 2021. Mol Ther. 29:744. PubMed\u003cbr\u003e\nRRID: AB_2565931 (BioLegend Cat. No. 141731) AB_2565932 (BioLegend Cat. No. 141732)\u003cbr\u003e\nStructure: Type I transmembrane protein, 175 kD, C-type lectin superfamily\u003cbr\u003e\nDistribution: Macrophages, dendritic cells, Langerhans cells, liver endothelial cells\u003cbr\u003e\nFunction: Pathogen recognition, endocytosis and phagocytosis, antigen presentation\u003cbr\u003e\nLigand\/Receptor: Antigen containing mannose, fucose, or an N-acetyl glucosamine\u003cbr\u003e\nCell Type: Dendritic cells, Endothelial cells, Langerhans cells, Macrophages\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501. 2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469. 3. Burgdorf S, et al. 2006. J. Immunol. 176:6770. 4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.\u003cbr\u003e\nGene ID: 17533\u003cbr\u003e\nUniProt: View information about CD206 on UniProt.org\u003cbr\u003e\nClone: C068C2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MMR (macrophage mannose receptor), MR (mannose receptor), MRC1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: Why is mouse CD206 stained intracellularly and not via surface staining?\u003cbr\u003e\nA: Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866086101161,"sku":"141731","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-141731","provider":"Iright","version":"1.0","type":"link"}