{"product_id":"biolegend-142504","title":"Biolegend, 142504, PE anti-mouse CD138 (Syndecan-1) Antibody, 200μg","description":"\u003cp\u003eCD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse mammary gland epithelial cell line NMuMG\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC) Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF) Li Q, et al. 2002. Cell 111:635. (IF, IHC) McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)\u003cbr\u003e\nProduct Citations: Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed Jash A, et al. 2016. J Immunol. 197: 1159 - 1168. PubMed Pandey SP, et al. 2022. Cell Host Microbe. 30:1003. PubMed Chen HY, et al. 2022. Elife. 11:. PubMed Kuang C, et al. 2023. Nat Commun. 14:2093. PubMed Grégoire C, et al. 2022. Immunity. 55:1216. PubMed Voss LF, et al. 2023. Front Immunol. 13:1021370. PubMed Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed Wang L, et al. 2019. Cancer Lett. 440-441:135. PubMed Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed Wang J, et al. 2021. ACS Nano. 15:14360. PubMed Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed Kim Y, et al. 2015. PLoS One. 10:120294. PubMed Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed Pan H, et al. 2020. Mol Psychiatry. . PubMed Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed Tu X, et al. 2022. Nat Commun. 13:6977. PubMed Yang Y, et al. 2021. Nat Commun. 12:525. PubMed Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed Utley A, et al. 2020. Cell Rep. 31:107815. PubMed Klessing S, et al. 2020. Vaccines (Basel). 8:. PubMed Chen M, et al. 2016. J Immunol . 194: 2607-15. PubMed Qi T, et al. 2020. Cell Rep. 33:108452. PubMed Liao T, et al. 2017. Front Immunol. 8:1334. PubMed Roy K, et al. 2019. Immunity. 50:616. PubMed Schoeler K, et al. 2019. FEBS J. 10.1111\/febs.14934. PubMed Cook KD, et al. 2015. Immunity. 43:703-714. PubMed Noviski M, et al. 2018. Elife. 7:e35074. PubMed Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed Li H, et al. 2020. Nature. 584:274. PubMed Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed Ribeiro de Almeida C, et al. 2018. Mol Cell. 70:650. PubMed Frost JN, et al. 2021. Med (N Y). 2:164. PubMed Cho S, et al. 2018. Nat Commun. 9:2757. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Schager AE, et al. 2018. MBio. 9:02379. PubMed Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed Zheng B, et al. 2021. Bioact Mater. 6:3879. PubMed van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed Che N, et al. 2021. Front Immunol. 12:626310. PubMed BJ L, et al. 2017. J Exp Med . 10.1084\/jem.20161461. PubMed Song W, et al. 2022. Immunity. 55:290. PubMed Steinmetz TD, et al. 2021. Autophagy. 17:2238. PubMed\u003cbr\u003e\nRRID: AB_10915989 (BioLegend Cat. No. 142503) AB_10916119 (BioLegend Cat. No. 142504)\u003cbr\u003e\nStructure: Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD\u003cbr\u003e\nFunction: Cell adhesion, cell motility, cell migration, cell proliferation\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Adhesion, Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Zong F, et al. 2011. PLoS ONE 6:e14816. 2. Yamashita Y, et al. 1999. J. Immunol. 162:5940. 3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.\u003cbr\u003e\nGene ID: 20969\u003cbr\u003e\nUniProt: View information about CD138 on UniProt.org\u003cbr\u003e\nClone: 281-2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Syndecan-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860883558569,"sku":"142504","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-142504","provider":"Iright","version":"1.0","type":"link"}