{"product_id":"biolegend-142523","title":"Biolegend, 142523, Brilliant Violet 421™ anti-mouse CD138 (Syndecan-1) Antibody, 50μg","description":"\u003cp\u003eCD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse mammary gland epithelial cell line NMuMG\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: µg sizes: 0.2 mg\/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For flow cytometric staining using the µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC) Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF) Li Q, et al. 2002. Cell 111:635. (IF, IHC) McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)\u003cbr\u003e\nProduct Citations: Hatzi K, et al. 2019. Nat Immunol. 20:86. PubMed Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed Daum P, et al. 2023. Front Immunol. 13:991347. PubMed Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Rivas MA, et al. 2021. Nat Immunol. 22:240. PubMed Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed Kodali S, et al. 2022. J Immunol. 208:1085. PubMed Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016\/j.jaut.2017.11.005. PubMed Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed Huang L, et al. 2015. J Immunol. 194:283. PubMed Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed Kenderes KJ, et al. 2018. Cell Rep. 24:824. PubMed Duan L, et al. 2021. Immunity. 54:2256. PubMed Qi T, et al. 2020. Cell Rep. 33:108452. PubMed Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed Mintz MA, et al. 2019. Immunity. 51:310. PubMed Ise W, et al. 2018. Immunity. 48:702. PubMed Glaros V, et al. 2021. Immunity. 54:2005. PubMed Béguelin W, et al. 2020. Cancer Cell. 37(5):655-673. PubMed Schoeler K, et al. 2019. FEBS J. 10.1111\/febs.14934. PubMed Reismann D, et al. 2017. Nat Commun.. 10.1038\/s41467-017-01538-9. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed Alameh MG, et al. 2021. Immunity. 54:2877. PubMed He Z, et al. 2016. Exp Hematol. 44:161-165. PubMed Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed Frost JN, et al. 2021. Med (N Y). 2:164. PubMed Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed Scally S, et al. 2017. Nat Commun. . 10.1038\/s41467-017-01924-3. PubMed Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed Viant C, et al. 2020. Cell. 183:1298. PubMed Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed Nascimento DC, et al. 2021. Immunity. :. PubMed Chen PM, et al. 2022. Sci Adv. 8:eabo4271. PubMed Trindade BC, et al. 2021. Immunity. 54:2273. PubMed Li X, et al. 2021. Front Immunol. 12:779560. PubMed Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed\u003cbr\u003e\nRRID: AB_11204257 (BioLegend Cat. No. 142507) AB_2565621 (BioLegend Cat. No. 142523) AB_11203544 (BioLegend Cat. No. 142508)\u003cbr\u003e\nStructure: Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD\u003cbr\u003e\nFunction: Cell adhesion, cell motility, cell migration, cell proliferation\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Adhesion, Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Zong F, et al. 2011. PLoS ONE 6:e14816. 2. Yamashita Y, et al. 1999. J. Immunol. 162:5940. 3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.\u003cbr\u003e\nGene ID: 20969\u003cbr\u003e\nUniProt: View information about CD138 on UniProt.org\u003cbr\u003e\nClone: 281-2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Syndecan-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860884967593,"sku":"142523","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-142523","provider":"Iright","version":"1.0","type":"link"}