{"product_id":"biolegend-144101","title":"Biolegend, 144101, Purified anti-mouse PIR-A\/B Antibody, 50μg","description":"\u003cp\u003ePaired-immunoglobulin-like receptor (PIR) is a type I transmembrane protein, containing six Ig-like domains. Two isoforms (PIR-A and PIR-B) have been reported for this molecule. PIR-A\/B is expressed on B cells, mast cells, macrophages, granulocytes, eosinophils, and dendritic cells. PIR-A requires association with FcRγc for its cell surface expression and to act as an activating receptor. The ligand for PIR-A is MHC class I. PIR-B is an inhibitory receptor with 3 ITIM motifs on its cytoplasmic tail; when phosphorylated, they recruit the tyrosine phosphatases PTPN6\/SHP-1 and PTPN11\/SHP-2. MHC class I, Nogo, MAG, and OMgp are the ligands for PIR-B.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant extracellular domains 1 and 2 of PIR-A\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F, WB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications for the relevant formats include: Western Blotting1 and immunohistochemical staining of frozen tissue sections3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kubagawa H, et al. 1999. J. Exp. Med. 189:309. (WB) Nakamura A, et al. 2004. Nat. Immunol. 5:623. (FC) Masuda K, et al. 2005. EMBO J. 24:4052. (IHC)\u003cbr\u003e\nProduct Citations: Shang P, et al. 2023. Nat Commun. 14:2615. PubMed\u003cbr\u003e\nRRID: AB_11204253 (BioLegend Cat. No. 144101)\u003cbr\u003e\nStructure: Transmembrane proteins, six Ig-like domains. PIR-A associates with FcRγc, doesn't have functional motifs on its cytoplasmic domain. PIR-B contains 3 ITIM motifs on its cytoplasmic domain.\u003cbr\u003e\nDistribution: B cells, macrophages, dendritic cells, mast cells, eosinophils.\u003cbr\u003e\nInteraction: PIR-A: FcRγcPIR-B: Lyn, PTPN6\/SHP-1, PTPN11\/SHP-2\u003cbr\u003e\nLigand\/Receptor: MHC class I, Nogo, MAG, OMgp.\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Eosinophils, Macrophages, Mast cells, Neutrophils\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: MHC Antigens, Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Matsushita H, et al. 2011. J. Biol. Chem. 286:25739. 2. Arita K, et al. 2011. J. Immunol. 186:7050. 3. Takai T, et al. 2011. J. Biomed. Biotechnol. 2011:275302. 4. Nakamura A, et al. 2004. Nat. Immunol. 5:623.\u003cbr\u003e\nGene ID: 1872218733\u003cbr\u003e\nUniProt: View information about PIR-A\/B on UniProt.org\u003cbr\u003e\nClone: 6C1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PIRA1, PIR-B Leukocyte immunoglobulin-like receptor 3, LIR-3\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862474051753,"sku":"144101","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-144101","provider":"Iright","version":"1.0","type":"link"}