{"product_id":"biolegend-145503","title":"Biolegend, 145503, PE anti-mouse CD185 (CXCR5) Antibody, 25μg","description":"\u003cp\u003eCD185 is also known as CXCR5. It is the receptor for chemokine CXCL13\/BLC, which is chemotactic for B cells.  CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow.  It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches.  Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: mCXCR5-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.\u003cbr\u003e\nProduct Citations: del Rio ML, et al. 2023. Front Immunol. 14:1113858. PubMed Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Chen Q, et al. 2022. Cell Rep. 39:110990. PubMed Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed Rios D, et al. 2015. Mucosal Immunol. 101038\/mi. PubMed Kurniawan H, et al. 2020. Cell Metabolism. 31(5):920-936. PubMed Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed Xing Y, et al. 2022. Cancer Lett. 529:100. PubMed Frost JN, et al. 2021. Med (N Y). 2:164. PubMed Tong D, et al. 2020. Protein Cell. 0.533333333. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed\u003cbr\u003e\nRRID: AB_2561967 (BioLegend Cat. No. 145503) AB_2561968 (BioLegend Cat. No. 145504)\u003cbr\u003e\nStructure: 42 kD, seven transmembrane protein\u003cbr\u003e\nDistribution: B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain\u003cbr\u003e\nFunction: B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma\u003cbr\u003e\nLigand\/Receptor: BLC\/CXCL13\u003cbr\u003e\nCell Type: B cells, T cells, Tfh\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532. 2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381. 3. Forster R, et al. 1994. Blood 84:830. 4. Forster R, et al. 1996. Cell 87:1037. 5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.\u003cbr\u003e\nGene ID: 12145\u003cbr\u003e\nUniProt: View information about CD185 on UniProt.org\u003cbr\u003e\nClone: L138D7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BLR1, GPCR6, MDR-15\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866117296297,"sku":"145503","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-145503","provider":"Iright","version":"1.0","type":"link"}