{"product_id":"biolegend-145505","title":"Biolegend, 145505, APC anti-mouse CD185 (CXCR5) Antibody, 25μg","description":"\u003cp\u003eCD185 is also known as CXCR5. It is the receptor for chemokine CXCL13\/BLC, which is chemotactic for B cells.  CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow.  It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches.  Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: mCXCR5-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.\u003cbr\u003e\nProduct Citations: Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed Kedmi R, et al. 2022. Nature. 610:737. PubMed Gao Y, et al. 2023. iScience. 26:106729. PubMed Zhao J, et al. 2021. Emerg Microbes Infect. 10:913. PubMed Mi Z, et al. 2021. Vaccines (Basel). 10: . PubMed Zander R, et al. 2022. Immunity. 55:475. PubMed Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed Essig K et al. 2017. Immunity. 47(6):1067-1082 . PubMed Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed Jing L, et al. 2022. Front Immunol. 13:864995. PubMed Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed Zhang X, et al. 2021. Front Immunol. 12:680068. PubMed Myers DR et al. 2019. Cell reports. 27(6):1858-1874 . PubMed Liao T, et al. 2017. Front Immunol. 8:1334. PubMed Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed Zhang YN, et al. 2020. EBioMedicine. 56:102819. PubMed Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed Yan J, et al. 2020. Cell Rep. 107820:31. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Chen C, et al. 2021. J Virol. 95:e0082921. PubMed He C, et al. 2022. Nat Commun. 13:5459. PubMed Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed Li X, et al. 2021. Front Immunol. 12:779560. PubMed\u003cbr\u003e\nRRID: AB_2561969 (BioLegend Cat. No. 145505) AB_2561970 (BioLegend Cat. No. 145506)\u003cbr\u003e\nStructure: 42 kD, seven transmembrane protein\u003cbr\u003e\nDistribution: B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain\u003cbr\u003e\nFunction: B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma\u003cbr\u003e\nLigand\/Receptor: BLC\/CXCL13\u003cbr\u003e\nCell Type: B cells, T cells, Tfh\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532. 2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381. 3. Forster R, et al. 1994. Blood 84:830. 4. Forster R, et al. 1996. Cell 87:1037. 5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.\u003cbr\u003e\nGene ID: 12145\u003cbr\u003e\nUniProt: View information about CD185 on UniProt.org\u003cbr\u003e\nClone: L138D7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BLR1, GPCR6, MDR-15\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866116870313,"sku":"145505","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-145505","provider":"Iright","version":"1.0","type":"link"}