{"product_id":"biolegend-148502","title":"Biolegend, 148502, Purified anti-mouse\/rat CD42d Antibody, 500μg","description":"\u003cp\u003eCD42d is an 83 kD surface glycoprotein that non-covalently associates with GPIb and GPIX to form a receptor complex for von Willebrand factor on megakaryocytes and resting platelets. Binding sites for von Willebrand factor and thrombin have been localized to the GPIba chain of the GPI-b-V-IX complex. Platelet activation with thrombin cleaves the GPI-b-V-IX complex to release a 69 kD soluble fragment. Presence of the GPI-b-V-IX complex is important in Bernard-Soulier syndrome, a rare bleeding disorder.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Mouse platelets\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for relevant formats) include: immunoprecipitation1,3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Takada K, et al. 1995. Hybridoma. 14:361. (IP) Saito M, et al. 1996. Stem Cells. 14:124. (FC) Sato N, et al. 2000. Hybridoma. 19:455. (IP)\u003cbr\u003e\nProduct Citations: Yeung AK, et al. 2020. Blood Adv. 4:6204. PubMed\u003cbr\u003e\nRRID: AB_2564114 (BioLegend Cat. No. 148501)\u003cbr\u003e\nStructure: 83 kD heterodimer composed of 2 polypeptide chains, an α chain (GP1BA) and a β chain (GP1BB).\u003cbr\u003e\nDistribution: Resting platelets and megakaryocytes.\u003cbr\u003e\nFunction: CD42d is a component of the GPIb-V-IX complex, functions as a vWF receptor, and mediates vWF-dependent platelet adhesion to blood vessels.\u003cbr\u003e\nLigand\/Receptor: CD42d.\u003cbr\u003e\nCell Type: Megakaryocytes, Platelets\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Sivaraman B, et al. 2011. Biomaterials. 32:5365. 2. Berger G, et al. 1996. Blood. 87:1385. 3. Ravanat C, et al. 1997. Blood. 89:3253. 4. Andrews RK, et al. 1998. Biochemistry. 37:638. 5. Inoue O, et al. 2008. 283:16279. 6. Hickey MJ, et al. 1993. Proc. Natl. Acad. Sci. 90:8327. 7. Shimomura T, et al. 1990. Blood. 75:2349. 8. Wu G, et al. 1997. Blood. 90:2660.\u003cbr\u003e\nGene ID: 14729\u003cbr\u003e\nUniProt: View information about CD42d on UniProt.org\u003cbr\u003e\nClone: 1C2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Platelet glycoprotein V, GPV, platelet gpV, CD42d antigen, CD42d\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860847841449,"sku":"148502","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-148502","provider":"Iright","version":"1.0","type":"link"}