{"product_id":"biolegend-149308","title":"Biolegend, 149308, PerCP\/Cyanine5.5 anti-mouse CD182 (CXCR2) Antibody, 100μg","description":"\u003cp\u003eCD182, also known as CXCR2, is a G-protein coupled receptor with 7 transmembrane regions that is involved in chemotaxis, neutrophil activation and angiogenesis. CXCR2 is expressed by neutrophils, basophils, subset of T cells, monocytes, macrophages, NK and NKT cells. Its ligands are CXCL1, CXCL2, CXCL3 and CXCL5.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse CXCR2-transfected cells.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nProduct Citations: Mollaoglu G et al. 2018. Immunity. 49(4):764-779 . PubMed Yao ME, et al. 2022. Evid Based Complement Alternat Med. 2022:7784148. PubMed Adrover JM et al. 2019. Immunity. 50(2):390-402 . PubMed Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed Dudeck J, et al. 2021. Immunity. 54(3):468-483.e5. PubMed\u003cbr\u003e\nRRID: AB_2565695 (BioLegend Cat. No. 149307) AB_2565696 (BioLegend Cat. No. 149308)\u003cbr\u003e\nStructure: G-protein coupled receptor, 7 transmembrane regions.\u003cbr\u003e\nDistribution: Neutrophils, basophils, subset of T cells, monocytes, macrophages, and natural killer cells (NK).\u003cbr\u003e\nFunction: Chemotaxis, neutrophil activation, and angiogenesis\u003cbr\u003e\nLigand\/Receptor: CXCL1, CXCL2, CXCL3, and CXCL5.\u003cbr\u003e\nCell Type: Neutrophils\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Spaan AN, et al. 2014. Nat. Commun. 5:5438. 2. Natsuaki Y, et al. 2014. Nat. Immunol. 11:1064. 3. Hsieh CY, et al. 2014. J. Immunol. 193:3693. 4. Diana J, and Lehuen A. 2014. EMBO Mol. Med. 6:1090. 5. Stoolman JS, et al. 2014. J. Immunol. 193:564.\u003cbr\u003e\nGene ID: 12765\u003cbr\u003e\nUniProt: View information about CD182 on UniProt.org\u003cbr\u003e\nClone: SA044G4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL-8RB, GRO\/MGSA, Cmkar2\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866280054953,"sku":"149308","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-149308","provider":"Iright","version":"1.0","type":"link"}