{"product_id":"biolegend-149529","title":"Biolegend, 149529, Brilliant Violet 711™ anti-mouse CD16.2 (FcγRIV) Antibody, 50μg","description":"\u003cp\u003eFcγRIV, also known as CD16.2, is an intermediate-affinity activating receptor for IgG2a and IgG2b. CD16.2 is the mouse homolog of human FcγRIIIA. CD16.2 is a low-affinity IgE receptor for all allotypes and the ligation of FcγRIV by antigen-IgE immune complexes promotes macrophage-mediated phagocytosis and is involved in lung inflammation.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: FCγR4 —EC domain fusion with IgG1 Fc\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710\/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats of this clone) include: blocking of Fc?RIV function1 and inhibition of immune complex binding1,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Mancardi DA, et al. 2008. J. Clin. Invest 118:3738. (FC, Block) Nimmerjahn F, et al. 2005. Immunity 23:41.\u003cbr\u003e\nRRID: AB_2687226 (BioLegend Cat. No. 149529)\u003cbr\u003e\nStructure: 22.4 kD\u003cbr\u003e\nDistribution: Expressed on peripheral blood leukocytes, spleen, colon, bone marrow, and the intestine\u003cbr\u003e\nFunction: Activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory.\u003cbr\u003e\nLigand\/Receptor: IgG2a and IgG2b\u003cbr\u003e\nCell Type: B cells, Leukocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nAntigen References: Mechetina LV, et al. 2002. Immunogenetics. 54:463-8. Nimmerjahn F, et al. 2005. Immunity. 23:41-51. Seeling M, et al. 2013. Proc. Natl. Acad. Sci. 110:10729-34.\u003cbr\u003e\nGene ID: 246256\u003cbr\u003e\nUniProt: View information about CD16.2 on UniProt.org\u003cbr\u003e\nClone: 9E9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FcgRIV, Fcgr4, Fcrl3, CD16-2, FcgRIV, Fcgr3a, FcgammaRIV, 4833442P21Rik, Fc gamma RIV\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862510194857,"sku":"149529","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-149529","provider":"Iright","version":"1.0","type":"link"}