{"product_id":"biolegend-152209","title":"Biolegend, 152209, PE\/Dazzle™ 594 anti-mouse CD34 Antibody, 25μg","description":"\u003cp\u003eCD34, also known as gp105-120, is a highly glycosylated type I monomeric sialomucin-like glycophophoprotein with an approximate molecular weight of 105-120 kD, with two generated by alternative splicing. CD34 is expressed on hematopoietic progenitors, endothelial cells, brain, and testis. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands, and mediates the attachment of stem cells to the extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse CD34 transfected cells.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Dazzle™ 594 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE\/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: This antibody (clone SA376A4) is useful for staining mouse CD34+ hematopoietic stem cell but it does not stain NIH-3T3 cells and the staining of Bend.3 cells is dimmer compared to other mouse CD34 antibodies, probably due to this antibody recognizes a different epitope than the other monoclonal antibodies.\u003cbr\u003e\nRRID: AB_2734218 (BioLegend Cat. No. 152209) AB_2734219 (BioLegend Cat. No. 152210)\u003cbr\u003e\nStructure: Type I monomeric glycophophoprotein with an approximate molecular weight of 105-120 kD.\u003cbr\u003e\nDistribution: Hematopoietic progenitors, endothelial cells, brain and testis tissues.\u003cbr\u003e\nFunction: Cell adhesion and homing.\u003cbr\u003e\nInteraction: PKC.\u003cbr\u003e\nLigand\/Receptor: L-selectin and E-selectin.\u003cbr\u003e\nCell Type: Endothelial cells, Hematopoietic stem and progenitors\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Garlanda C, et al. 1997. Eur J Cell Biol. 73:368-77. 2. Brown J, et al. 1991. Int Immunol. 3:175. 3. Suda J, et al. 1992. Blood. 79:2288-95. 4. Baumhueter S, et al. 1994. Blood. 84:2554.\u003cbr\u003e\nGene ID: 12490\u003cbr\u003e\nUniProt: View information about CD34 on UniProt.org\u003cbr\u003e\nClone: SA376A4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: My10, gp105-120, mucosialin\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866293391529,"sku":"152209","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-152209","provider":"Iright","version":"1.0","type":"link"}