{"product_id":"biolegend-154305","title":"Biolegend, 154305, PE anti-mouse Perforin Antibody, 25μg","description":"\u003cp\u003ePerforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse perforin transfected cells.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Clone S16009A reacts with perforin from both C57BL\/6 and BALB\/c strains.\u003cbr\u003e\nProduct Citations: Wang M, et al. 2022. Adv Sci (Weinh). 9:e2202914. PubMed Pan Y, et al. 2023. Adv Sci (Weinh). 10:e2206792. PubMed Li H, et al. 2022. Cancer Cell. 40:36. PubMed Jia JJ, et al. 2022. J Leukoc Biol. 112:157. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Liu L, et al. 2022. Nat Commun. 13:6740. PubMed Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed Xu W 2023. Cell Reports. 42(1):111987. PubMed Wang W, et al. 2022. World J Gastrointest Oncol. 14:1124. PubMed Macchi C, et al. 2022. iScience. 25:104435. PubMed Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed Goggi JL, et al. 2020. Mol Imaging Biol. 22:1392. PubMed Wu J, et al. 2020. Immunity. 53:115. PubMed Chandana M, et al. 2022. Nat Commun. 13:4028. PubMed Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed Shen H, et al. 2022. Nat Commun. 13:5013. PubMed\u003cbr\u003e\nRRID: AB_2721638 (BioLegend Cat. No. 154305) AB_2721639 (BioLegend Cat. No. 154306)\u003cbr\u003e\nStructure: 70 kD Protein that oligomerizes for pore formation.\u003cbr\u003e\nDistribution: CTL, NK (Cytoplasmic Granules)\u003cbr\u003e\nFunction: Target cell lysis\u003cbr\u003e\nCell Type: NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: Podack ER, et al. 1988. Immunol. Rev. 103:203. Konjar S, et al. 2010. Immunology. 131:257. Trapani JA, et al. 2002. Nat. Rev. Immunol. 2:735. Osinska I, et al. 2014. Cent. Eur. J. Immnol. 39:109.\u003cbr\u003e\nGene ID: 18646\u003cbr\u003e\nUniProt: View information about Perforin on UniProt.org\u003cbr\u003e\nClone: S16009A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Prf1, Pfp, Pfn, Pfn-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866222416041,"sku":"154305","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-154305","provider":"Iright","version":"1.0","type":"link"}