{"product_id":"biolegend-154311","title":"Biolegend, 154311, Pacific Blue™ anti-mouse Perforin Antibody, 25μg","description":"\u003cp\u003ePerforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse perforin transfected cells.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Clone S16009A reacts with perforin from both C57BL\/6 and BALB\/c strains.\u003cbr\u003e\nRRID: AB_2922481 (BioLegend Cat. No. 154311) AB_2922481 (BioLegend Cat. No. 154312)\u003cbr\u003e\nStructure: 70 kD Protein that oligomerizes for pore formation.\u003cbr\u003e\nDistribution: CTL, NK (Cytoplasmic Granules)\u003cbr\u003e\nFunction: Target cell lysis\u003cbr\u003e\nCell Type: NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: Podack ER, et al. 1988. Immunol. Rev. 103:203. Konjar S, et al. 2010. Immunology. 131:257. Trapani JA, et al. 2002. Nat. Rev. Immunol. 2:735. Osinska I, et al. 2014. Cent. Eur. J. Immnol. 39:109.\u003cbr\u003e\nGene ID: 18646\u003cbr\u003e\nUniProt: View information about Perforin on UniProt.org\u003cbr\u003e\nClone: S16009A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Prf1, Pfp, Pfn, Pfn-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866222645417,"sku":"154311","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-154311","provider":"Iright","version":"1.0","type":"link"}