{"product_id":"biolegend-157505","title":"Biolegend, 157505, PE anti-mouse CD40 Antibody, 25μg","description":"\u003cp\u003eCD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34 + hematopoietic progenitors. CD40 regulates B cell development\/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant mouse CD40 – Fc fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The FGK45 antibody can block the binding of HM40-3 clone. The FGK45 antibody can also stimulate mouse B cell proliferation.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Andersson J, et al. 1996. Immunity 5:319-330. Melief CJ, et al. 1998. Nature 393(6684):480-3. Londei M, et al. 2000. Nat Med 6(6):673-9. Tominaga T, et al. 2015. Neuro Oncol 17(11):1453-62.\u003cbr\u003e\nProduct Citations: Bhaskar A, et al. 2023. iScience. 26:106644. PubMed Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed\u003cbr\u003e\nRRID: AB_2832552 (BioLegend Cat. No. 157505) AB_2860731 (BioLegend Cat. No. 157506)\u003cbr\u003e\nStructure: 48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.\u003cbr\u003e\nDistribution: Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.\u003cbr\u003e\nFunction: Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.\u003cbr\u003e\nInteraction: TRAF molecules\u003cbr\u003e\nLigand\/Receptor: CD154 (CD40L)\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, Dendritic cells, Macrophages\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.\u003cbr\u003e\nGene ID: 21939\u003cbr\u003e\nUniProt: View information about CD40 on UniProt.org\u003cbr\u003e\nClone: FGK45\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Bp50, TNFRSF5\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863997993129,"sku":"157505","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-157505","provider":"Iright","version":"1.0","type":"link"}