{"product_id":"biolegend-158605","title":"Biolegend, 158605, APC anti-mouse IRF5 Antibody, 25μg","description":"\u003cp\u003eThe interferon regulatory factor (IRF) family of transcription factors comprises nine members with conserved domains. IRF5 mediates the induction of proinflammatory cytokine, such as IL-6 and TNFa. IRF5 is highly expressed in monocytes, macrophages, B cells, and dendritic cells. Expression of IRF5 is associated with many autoimmune diseases.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse IRF5 recombinant protein (149-400 a.a.)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: This clone was tested for ICC using RAW264.7 cells fixed with 4% PFA and permeabilized with either Triton X-100 or methanol. While both methods were compatible with IRF5 staining, the signal was weak and did not meet our sensitivity standards. This clone does not recognize human IRF5. For detection of human IRF5, please use BioLegend clone 11F4A09 (Cat. No. 652602). During product development testing of this clone for western blot, a ~37 kD protein of unknown origin was faintly detected. This protein may represent a minor IRF5 degradation product.\u003cbr\u003e\nRRID: AB_2924490 (BioLegend Cat. No. 158605) AB_2924490 (BioLegend Cat. No. 158606)\u003cbr\u003e\nStructure: 497 a.a. (56 kD)\u003cbr\u003e\nDistribution: Monocytes, macrophages, B cells, dendritic cells\u003cbr\u003e\nFunction: Involved in IFNa and IFNb induction in response to viral infection\u003cbr\u003e\nCell Type: B cells, Macrophages, Monocytes\u003cbr\u003e\nBiology Area: Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: Adaptor Proteins, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: Kobayashi S, et al. 2019. J Immunol. 203(6):1447-1456. Nie S, et al. 2019. Clin Immunol. 207:24-35. Pandey SP, et al. 2019. Mucosal Immunol. 12(4):1065.\u003cbr\u003e\nGene ID: 27056\u003cbr\u003e\nUniProt: View information about IRF5 on UniProt.org\u003cbr\u003e\nClone: W16007B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IRF5, Irf5, IRF-5, Interferon regulatory factor 5\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866300895401,"sku":"158605","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-158605","provider":"Iright","version":"1.0","type":"link"}