{"product_id":"biolegend-200201","title":"Biolegend, 200201, Purified anti-rat CD80 Antibody, 50μg","description":"\u003cp\u003eCD80 is a 60 kD member of the immunoglobulin superfamily also known as B7-1, B7, and BB1. It is expressed on antigen presenting cells and activated T and B cells, and is a ligand for CD28 and CD152 (CTLA-4). CD80\/CD28 interactions are important for T and B cell co-stimulation, while CD80\/CD152 lead to the inhibition of T cell responses. The 3H5 antibody can block the co-stimulatory activity of CD80.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: HTLV-1-transformed Lewis-S1 rat T cell line\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality testedIHC-F, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of ligand binding1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200208).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Maeda, K., et al. 1997. Int. Immunol. 9:993. (IP, Block) Bluestone, J.A., 1995. Immunity 2:555. (IP, Block) Damoiseaux, J.G., et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC) Ho, CH., et al. 2007. J. Virol. 81:4837. PubMed Ho, C., et al. 2009. Carcinogenesis 30:1443. PubMed\u003cbr\u003e\nProduct Citations: Ho C, et al. 2009. Carcinogenesis. 30:1443. PubMed Ho C, et al. 2007. J Virol. 81:4837. PubMed\u003cbr\u003e\nRRID: AB_313843 (BioLegend Cat. No. 200202)\u003cbr\u003e\nStructure: Ig superfamily, 60 kD\u003cbr\u003e\nDistribution: Antigen presenting cells, activated B cells and T cells\u003cbr\u003e\nFunction: T and B cell costimulation\u003cbr\u003e\nLigand\/Receptor: CD28, CD152 (CTLA-4)\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Barclay, A.N. et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Linsley, P.S., et al. 1991. J. Exp. Med. 174:561. 3. Salomon, B., et al. 2001. Ann. Rev. Immunol. 19:225.\u003cbr\u003e\nGene ID: 25408\u003cbr\u003e\nUniProt: View information about CD80 on UniProt.org\u003cbr\u003e\nClone: 3H5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B7-1, B7, BB1, Ly-53\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862400192681,"sku":"200201","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-200201","provider":"Iright","version":"1.0","type":"link"}