{"product_id":"biolegend-202403","title":"Biolegend, 202403, Biotin anti-rat CD54 Antibody, 100μg","description":"\u003cp\u003eCD54 is an 85 kD protein also known as ICAM-1. It is a member of the immunoglobulin superfamily that is expressed on high endothelial venules in lymphoid tissue, peripheral blood monocytes, dendritic cells, granulocytes and thymic stromal cells. CD54 is weakly expressed on T and B cells, although expression can be increased by activation (especially on endothelial cells). ICAM-1 is an important protein in cell adhesion, inflammation and immune responses. CD54 has been shown to bind to CD11a\/CD18 (LFA-1), CD11b\/CD18, CD11c\/CD18, CD43, fibrinogen, and hyaluronan. The 1A29 antibody has been reported to block T cell aggregation and binding of lymphocytes to HEV-derived endothelial cells and purified ICAM-1 protein.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: HEV-derived cell line Ax\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry1,5 of acetone-fixed frozen sections and STUF-treated paraffin-embedded sections, immunoprecipitation2, in vitro and in vivo blocking of PMA-induced T cell blast aggregation and adhesion to HEV-derived endothelial cells and purified ICAM-1 protein2,3,4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Clark WM, et al. 1995. Mol. Chem. Neuropathol. 26:213. (IHC) Tamatani T, et al. 1990. Int. Immunol. 2:165. (IP) Tamatani T, et al. 1991. Eur. J. Immunol. 21:627. Willenborg DO, et al. 1996. J. Immunol. 157:1973. McKechnie NM, et al. 2002. IVOS 43:411. (IHC)\u003cbr\u003e\nProduct Citations: Sikder S, et al. 2020. J Leukoc Biol. 107:263. PubMed\u003cbr\u003e\nRRID: AB_2121900 (BioLegend Cat. No. 202403)\u003cbr\u003e\nStructure: Ig superfamily, 85 kD\u003cbr\u003e\nDistribution: High endothelial venules in lymphoid tissues, peripheral blood monocytes, dendritic cells, granulocytes, thymic stromal cells and weakly on T and B cells. Upregulated on activated cells and endothelial cells.\u003cbr\u003e\nFunction: Cellular adhesion, inflammation, immune reactions\u003cbr\u003e\nLigand\/Receptor: CD11a\/CD18 (LFA-1), CD11b\/CD18, CD11c\/CD18, CD43, fibrinogen, hyaluronan\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Monocytes, T cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Tamatani T, et al. 1990. Int. Immunol. 2:165. 2. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627. 3. Turunen JP, et al. 1993. Scand. J. Immunol. 37:282.\u003cbr\u003e\nGene ID: 15894\u003cbr\u003e\nUniProt: View information about CD54 on UniProt.org\u003cbr\u003e\nClone: 1A29\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: ICAM-1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860833521833,"sku":"202403","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-202403","provider":"Iright","version":"1.0","type":"link"}