{"product_id":"biolegend-250302","title":"Biolegend, 250302, Purified anti-marmoset CD8 Antibody, 100μg","description":"\u003cp\u003eCD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a\/a) or heterodimer (CD8a\/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α 3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Common Marmoset\u003cbr\u003e\nReported Reactivity: Squirrel Monkey\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Marmoset mononuclear cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F, IP, WB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The 6F10 antibody has been reported to cross-react with lymphocytes from squirrel monkeys but not from cynomolgus monkeys or humans. Additional reported applications for the relevant formats include: iimmunohistochemical staining of frozen spleen sections1, Western blotting1, immunoprecipitation1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ito R, et al. 2008. Immunol. Lett. 121:116. (IHC, IP, WB)\u003cbr\u003e\nRRID: AB_2562121 (BioLegend Cat. No. 250302)\u003cbr\u003e\nStructure: Ig superfamily, homodimer or heterodimer with CD8β\u003cbr\u003e\nDistribution: Majority of thymocytes, T cell subset, NK cells\u003cbr\u003e\nFunction: MHC class I co-receptor, thymic differentiation, T cell activation\u003cbr\u003e\nLigand\/Receptor: MHC Class I molecules\u003cbr\u003e\nCell Type: NK cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.\u003cbr\u003e\nGene ID: 100387674\u003cbr\u003e\nUniProt: View information about CD8 on UniProt.org\u003cbr\u003e\nClone: 6F10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T8, Leu2\u003cbr\u003e\nIsotype: Mouse IgG1\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862479392937,"sku":"250302","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-250302","provider":"Iright","version":"1.0","type":"link"}