{"product_id":"biolegend-260418","title":"Biolegend, 260418, GMP APC anti-human CD69 Antibody, 100tests","description":"\u003cp\u003eCD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4 + T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nReactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w\/v) BSA (origin USA) and a stabilizer.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 100 µg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC) Kim JR, et al. 2005. BMC Immunol. 6:3. (IF) Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nDisclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products\u003cbr\u003e\nStructure: C-type lectin, type II glycoprotein, 28\/32 kD\u003cbr\u003e\nDistribution: Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells\u003cbr\u003e\nFunction: Lymphocyte, monocyte, and platelet activation, NK cell killing\u003cbr\u003e\nCell Type: B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Testi R, et al. 1994. Immunol. Today 15:479.\u003cbr\u003e\nGene ID: 969\u003cbr\u003e\nUniProt: View information about CD69 on UniProt.org\u003cbr\u003e\nClone: FN50\u003cbr\u003e\nWorkshop: IV A91\u003cbr\u003e\nOther Names: Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866522603689,"sku":"260418","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-260418","provider":"Iright","version":"1.0","type":"link"}