{"product_id":"biolegend-260466","title":"Biolegend, 260466, GMP PE\/Cyanine5 anti-human CD19 Antibody, 100tests","description":"\u003cp\u003eCD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.\u003cbr\u003e\n100tests\u003cbr\u003e\nReactivity: Human\u003cbr\u003e\nReported Reactivity: Chimpanzee\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w\/v) BSA (origin USA) and a stabilizer.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine5 under optimal conditions.\u003cbr\u003e\nConcentration: 25 µg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 \u0026amp; 302268). Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Bradbury L, et al. 1993. J. Immunol. 151:2915. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC) Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG) Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)\u003cbr\u003e\nDisclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products\u003cbr\u003e\nStructure: Ig superfamily, type I transmembrane glycoprotein, 95 kD\u003cbr\u003e\nDistribution: B lineage (except plasma cells), follicular dendritic cells\u003cbr\u003e\nFunction: B cell activation and differentiation\u003cbr\u003e\nLigand\/Receptor: Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Tedder T, et al. 1994. Immunol. Today 15:437. 2. Bradbury L, et al. 1993. J. Immunol. 151:2915.\u003cbr\u003e\nGene ID: 930\u003cbr\u003e\nUniProt: View information about CD19 on UniProt.org\u003cbr\u003e\nClone: HIB19\u003cbr\u003e\nWorkshop: V CD19.11\u003cbr\u003e\nOther Names: B4\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866523881641,"sku":"260466","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-260466","provider":"Iright","version":"1.0","type":"link"}