{"product_id":"biolegend-285250","title":"Biolegend, 285250, Spark Blue™ 550 anti-human TCR Vα7.2 (Flexi-Fluor™) Antibody, 50μg","description":"\u003cp\u003eThe 3C10 antibody recognizes the Vα7.2 T cell antigen receptor (TCR) α-chain segment which, joined with the Jα33 segment, constitutes an invariant TCR that is a characteristic of the mucosal-associated invariant T cells (MAIT cells). MAIT cells are restricted by a nonpolymorphic class Ib major histocompatibility complex (MHC) molecule, MHC-related molecule 1 (MR1). MAIT cells are present in human blood (1-8% of T cells), mesenteric lymph nodes, liver, and intestinal mucosa.  MAIT cells play a role in detecting and fighting off microbial infections.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant TCR\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC\u003cbr\u003e\nRecommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg\/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg\/mL. For example, make five 1:1 serial dilutions of the 0.2 mg\/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells\/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. * Spark Blue™ 550 has a maximum excitation of 516 nm and a maximum emission of 540 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)3,4.\u003cbr\u003e\nAdditional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Martin E, et al. 2009. PLoS Biol. 7:525. Wakao H, et al. 2013. Cell Stem Cell 12:1. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nRRID: AB_3662554 (BioLegend Cat. No. 285250)\u003cbr\u003e\nStructure: Invariant T cell antigen receptor (TCR) α-chain (iTCRa), composed of the invariant α-chain variable region 7.2 (iVα7.2) segment in humans\u003cbr\u003e\nDistribution: Mucosal-associated invariant T (MAIT) cells and conventional T cells\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immunology\u003cbr\u003e\nMolecular Family: TCRs\u003cbr\u003e\nAntigen References: 1. Le Bourhis L, et al. 2010. Nat. Immunol. 11:701.\u003cbr\u003e\nGene ID: 28692\u003cbr\u003e\nUniProt: View information about TCR Valpha7.2 on UniProt.org\u003cbr\u003e\nClone: 3C10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell antigen receptor Vα7.2, TRAV1-2, T cell receptor alpha variable 1-2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What are Flexi-Fluors?\u003cbr\u003e\nA: Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.\u003cbr\u003e\nQ: How quickly will I receive my order?\u003cbr\u003e\nA: We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.\u003cbr\u003e\nQ: How are Flexi-Fluors different from regular catalog products?\u003cbr\u003e\nA: Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.\u003cbr\u003e\nQ: How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?\u003cbr\u003e\nA: Flexi-Fluors are provided at a standard 0.2 mg\/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg\/mL. \u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eFor example, make five 1:1 serial dilutions of your 0.2 mg\/mL antibody.  Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells\/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.\u003cbr\u003e\nQ: I can’t find the antibody-dye combination that I need. When will it be available?\u003cbr\u003e\nA: We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.\u003cbr\u003e\nQ: I need help to validate the performance of my Flexi-Fluor. Who should I contact?\u003cbr\u003e\nA: Please get in touch with Technical Service for assistance.\u003cbr\u003e\nQ: Can I order more than 50 μg of a Flexi-Fluor?\u003cbr\u003e\nA: Yes, you can order multiple vials of the same Flexi-Fluor products.  We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.\u003cbr\u003e\nQ: What is the expiration date of my Flexi-Fluor?\u003cbr\u003e\nA: Expiration dates can be found on the vial label or by using our CoA lookup tool.\u003cbr\u003e\nQ: What if my requested antibody-dye combination doesn't perform as expected?\u003cbr\u003e\nA: Flexi-Fluor™ products have been carefully selected by our scientists for their excellent conjugation properties and broad compatibility. In the rare case that an antibody-dye combination does not perform as expected due to the unique characteristics of a specific pairing, please contact our Technical Service team. We will work with you to identify alternative options to meet your panel requirements.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862389051561,"sku":"285250","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-285250","provider":"Iright","version":"1.0","type":"link"}