{"product_id":"biolegend-300411","title":"Biolegend, 300411, APC anti-human CD3 Antibody, 25tests","description":"\u003cp\u003eCD3ε is a 20 kD chain of the CD3\/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α\/β or γ\/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Chimpanzee\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: µg sizes: 0.2 mg\/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt; 0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP) Graves J, et al. 1991. J. Immunol. 146:2102. (Activ) Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ) Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC) Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ) Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC) Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC) Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Van Dongen JJM, et al. 1988. Blood 71:603. (WB) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC) Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed Li J, et al. 2015. Cancer Res. 75:508. PubMed Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Crawford MP, et al. 2020. Proc Natl Acad Sci U S A. 117:19408. PubMed Toepfner N et al. 2018. eLife. 7 pii: e29213. PubMed Zhang Y, et al. 2023. Heliyon. 9:e14823. PubMed Crawford MP, et al. 2023. PLoS One. 18:e0285166. PubMed Jiang D, et al. 2023. Nat Commun. 14:3642. PubMed Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed Vazquez-Lombardi R, et al. 2022. Immunity. 55:1953. PubMed Wheeler MM, et al. 2022. Nat Commun. 13:7592. PubMed Ye L, et al. 2022. Cell Metab. 34:595. PubMed Yen M, et al. 2022. Cell. 185:1414. PubMed Martin–Fernandez M, et al. 2020. Cell Reports. 31(6):107633. PubMed Mayassi T, et al. 2019. Cell. 176:967. PubMed Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed Gutzeit C, et al. 2014. J Immunol. 192:5852. PubMed Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed Sun L, et al. 2020. J Diabetes Res. 2020:2583257. PubMed Ogishi M, et al. 2021. Nat Med. 27:1646. PubMed Wang C, et al. 2021. Cell Stem Cell. . PubMed Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed Paris O, et al. 2022. Front Immunol. 13:975910. PubMed Lichtenegger FS, et al. 2018. Front Immunol. 9:385. PubMed Constantinescu-Bercu A, et al. 2020. eLife. 9:00. PubMed Gerstner S, et al. 2016. J Leukoc Biol. 100(6):1297-1310. PubMed Levring TB, et al. 2019. Sci Rep. 9:16725. PubMed Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed Lu P, et al. 2021. Blood Cancer J. 11:39. PubMed Helmink BA, et al. 2020. Nature. 577:549. PubMed Wu D, et al. 2020. Biomark Res. 8:3. PubMed Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed Huang GL, et al. 2020. PLoS One. 15:e0241421. PubMed Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed Hu H, et al. 2022. Exp Ther Med. 24:487. PubMed Jing L, et al. 2020. Cancer Immunol Res. 8:648. PubMed Reinfeld BI, et al. 2021. Nature. 593:282. PubMed Meraviglia S, et al. 2017. Oncoimmunology. 6:e1347742. PubMed Fujii S, et al. 2019. Stem Cells Dev. 28:28. PubMed Hannon E, et al. 2021. PLoS Genet. e1009443:17. PubMed Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed Kim JR, et al. 2005. BMC Immunol. 2005,6:3. PubMed Ling X, et al. 2022. STAR Protoc. 3:101321. PubMed Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed Zhang B, et al. 2020. Cell Chemical Biology. 28(1):60-69.e7. PubMed Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed Barresi V, et al. 2020. J Clin Med. 9:00. PubMed Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed Morton B, et al. 2021. Nat Commun. 12:3554. PubMed Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed Singh KS, et al. 2021. Nature. 589:597. PubMed Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed Pierini S, et al. 2020. JCI Insight. 5:00. PubMed Ajith A, et al. 2019. FASEB J. 33:5220. PubMed Headland S, et al. 2014. Sci Rep. 4:5237. PubMed Giron LB, et al. 2020. EBioMedicine. 73.94791667. PubMed Lo Presti E, et al. 2020. J Leukoc Biol. 108:749. PubMed Siegers G, et al. 2011. PLoS One. 6:e16700. PubMed Anderson NR, et al. 2019. Cell Adh Migr. 13:163. PubMed\u003cbr\u003e\nRRID: AB_314065 (BioLegend Cat. No. 300411) AB_314066 (BioLegend Cat. No. 300412) AB_2564151 (BioLegend Cat. No. 300458) AB_2562045 (BioLegend Cat. No. 300439)\u003cbr\u003e\nStructure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α\/β or γ\/δ) forms CD3\/TCR complex, 20 kD\u003cbr\u003e\nDistribution: Mature T and NK T cells, thymocyte differentiation\u003cbr\u003e\nFunction: Antigen recognition, signal transduction, T cell activation\u003cbr\u003e\nLigand\/Receptor: Peptide antigen bound to MHC\u003cbr\u003e\nCell Type: NKT cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, TCRs\u003cbr\u003e\nAntigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.\u003cbr\u003e\nGene ID: 916\u003cbr\u003e\nUniProt: View information about CD3 on UniProt.org\u003cbr\u003e\nClone: UCHT1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: III 471\u003cbr\u003e\nOther Names: T3, CD3ε\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864073818281,"sku":"300411","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-300411","provider":"Iright","version":"1.0","type":"link"}