{"product_id":"biolegend-301425","title":"Biolegend, 301425, Ultra-LEAF™ Purified anti-human CD11b (activated) Antibody, 100μg","description":"\u003cp\u003eThe CBRM1\/5 antibody reacts with an activated form of human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as α M integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β 2 (CD18) and is expressed on granulocytes, monocytes\/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b\/CD18 is critical for the transendothelial migration of monocytes and neutrophils as well as adhesion, phagocytosis, and neutrophil activation. CD11b\/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and factor X.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.\u003cbr\u003e\nEndotoxin Level: Less than 0.01 EU\/µg of the protein (\u0026lt; 0.001 ng\/µg of the protein) as determined by the LAL test.\u003cbr\u003e\nPreparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg\/mL and 3 mg\/mL. Older lots may have also been bottled at 1 mg\/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.\u003cbr\u003e\nApplication: FC - Quality testedIP, Block - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The CBRM1\/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize \"non-activated\" CD11b. The epitope recognized by CBRM1\/5 is contained in the I domain of the a chain. Clone CBRM1\/5 binds the I domain, close to the ligand binding site. It recognizes a conformational change of the integrin9. Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking cell adhesion to fibrinogen and ICAM-11. The CBRM1\/5 antibody binds specifically to the activated form of CD11b. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301408).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Diamond M, et al. 1993. J. Cell Biol. 120:545. (Block) Bryn T, et al. 2006. J. Immunol. 176:7361. PubMed Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074\/jbc.M611273200. Barthel SR,et al.2006. Am J. Respir Cell Mol Biol.35:378. PubMed Pillay J,et al. 2010. J. Leukocyte Biol. 88:211. PubMed Wilson RP, et al. 2011. Infect Immun. 79:830. PubMed Koleva RI, et al. 2012. Blood. 119:4878. PubMed. Eleftherious D, et al. 2012. Neurology. 79:2089. PubMed Oxvig C, et al. 1999. Proc. Natl. Acad. Sci. USA 96:2215. Muller-Edenborn B, et al. 2014. Br J Anaesth. PubMed Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed\u003cbr\u003e\nRRID: AB_2860762 (BioLegend Cat. No. 301425) AB_2860763 (BioLegend Cat. No. 301426)\u003cbr\u003e\nStructure: Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD\u003cbr\u003e\nDistribution: Granulocytes, monocytes\/macrophages, dendritic cells, NK cells, T cell subset, B cell subset\u003cbr\u003e\nFunction: Adhesion, phagocytosis, chemotaxis, neutrophil activation\u003cbr\u003e\nLigand\/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Stewart M, et al. 1995. Curr. Opin. Cell Biol. 7:690. 2. Petty H, et al. 1996. Immunol. Today 17:209.\u003cbr\u003e\nGene ID: 3684\u003cbr\u003e\nUniProt: View information about CD11b on UniProt.org\u003cbr\u003e\nClone: CBRM1\/5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: Do you guarantee that your antibodies are totally pathogen free?\u003cbr\u003e\nA: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?\u003cbr\u003e\nA: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?\u003cbr\u003e\nA: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.\u003cbr\u003e\nQ: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?\u003cbr\u003e\nA: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862973862057,"sku":"301425","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-301425","provider":"Iright","version":"1.0","type":"link"}