{"product_id":"biolegend-302119","title":"Biolegend, 302119, PerCP\/Cyanine5.5 anti-human CD18 Antibody, 25tests","description":"\u003cp\u003eCD18 is a 90-95 kD type I transmembrane protein also known as integrin β 2 subunit, LFA-1 β subunit, and β 2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Pig\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: inhibition of cell adhesion and migration3,4. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 302116) is recommended for functional assays.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto T, et al. 1997. Leucocyte Typing VI. Garland Press. London. Van Epps DE, et al. 1989. J. Immunol. 143:3207. (Block) Meerschaert J, et al. 1994. J. Immunol. 152:1915. (Block) Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074\/jbc.M611273200. Sommaggio R, et al. 2012. J. Immunol. 188:2075. PubMed Valenzuela NM, et al. 2013. J. Immunol. 190:6635. PubMed\u003cbr\u003e\nRRID: AB_2565586 (BioLegend Cat. No. 302119) AB_2565587 (BioLegend Cat. No. 302120)\u003cbr\u003e\nStructure: Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c, 90-95 kD\u003cbr\u003e\nDistribution: Leukocytes\u003cbr\u003e\nFunction: Adhesion and signaling in hematopoietic system\u003cbr\u003e\nLigand\/Receptor: ICAMs\u003cbr\u003e\nCell Type: Leukocytes, Tregs\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175. 2. Springer T. 1994. Cell 76:301.\u003cbr\u003e\nGene ID: 3689\u003cbr\u003e\nUniProt: View information about CD18 on UniProt.org\u003cbr\u003e\nClone: TS1\/18\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V AS162\u003cbr\u003e\nOther Names: Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864010412201,"sku":"302119","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-302119","provider":"Iright","version":"1.0","type":"link"}