{"product_id":"biolegend-302632","title":"Biolegend, 302632, Brilliant Violet 605™ anti-human CD25 Antibody, 100tests","description":"\u003cp\u003eCD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes\/macrophages. CD25 is also expressed on a subset of non-stimulated CD4 + T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610\/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications include: immunocytochemistry3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed\u003cbr\u003e\nProduct Citations: Beck C, et al. 2023. Biomedicines. 11:. PubMed Saxena V, et al. 2022. Cell Rep. 39:110727. PubMed AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed Panwar B, et al. 2021. Genome Res. 31:659. PubMed Szabo PA, et al. 2021. Immunity. 54(4):797-814.e6. PubMed Nicholas DA et al. 2019. Cell Metab. 30(3):447-461 . PubMed Richert-Spuhler LE, et al. 2021. Cell Reports Medicine. 2(6):100322. PubMed Sayin I, et al. 2018. J Exp Med. 7:40286. PubMed Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed Gudgeon N, et al. 2022. Cell Rep. 40:111193. PubMed Oh DY, et al. 2020. Cell. 181:1612. PubMed OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed Platten M, et al. 2021. Nature. 592:463. PubMed Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed Strobel L, et al. 2021. J Clin Med. 10:00. PubMed Luca VC, et al. 2020. PLoS One. 15:e0226928. PubMed Nasrallah R, et al. 2020. Nature. 583:447. PubMed Kim ML, et al. 2021. iScience. 24:103509. PubMed\u003cbr\u003e\nRRID: AB_11123913 (BioLegend Cat. No. 302631) AB_11218989 (BioLegend Cat. No. 302632)\u003cbr\u003e\nStructure: Type I transmembrane glycoprotein, 55 kD\u003cbr\u003e\nDistribution: Activated T cells and B cells, monocytes\/macrophages, Treg\u003cbr\u003e\nFunction: Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex\u003cbr\u003e\nLigand\/Receptor: IL-2\u003cbr\u003e\nCell Type: B cells, Macrophages, Monocytes, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681.\u003cbr\u003e\nGene ID: 3559\u003cbr\u003e\nUniProt: View information about CD25 on UniProt.org\u003cbr\u003e\nClone: BC96\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: V T-072\u003cbr\u003e\nOther Names: Low affinity IL-2R, IL-2R α chain, Tac, p55\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862904426665,"sku":"302632","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-302632","provider":"Iright","version":"1.0","type":"link"}