{"product_id":"biolegend-304905","title":"Biolegend, 304905, PE anti-human CD62P (P-Selectin) Antibody, 25tests","description":"\u003cp\u003eCD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Chimpanzee\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4 and in vitro blocking of adhesion of platelets1. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304947 \u0026amp; 304948).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Skinner M, et al. 1991. J. Biol. Chem. 266:5371. (Block) Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Yen YT, et al. 2006. J. Virol. 80:2684. Sato Y, et al. 2005. Blood 106:428. (IHC)\u003cbr\u003e\nProduct Citations: Buijsers B, et al. 2022. Front Immunol. 12:759570. PubMed Garcia-Larragoiti N, et al. 2021. J Thromb Haemost. 19:2572. PubMed Li L, et al. 2022. FEBS J. 289:2877. PubMed Kemble S, et al. 2022. Blood Adv. 6:2932. PubMed Li L, et al. 2023. Br J Pharmacol. 180:287. PubMed Cano-Mendez A, et al. 2022. Int J Mol Sci. 23: . PubMed Machata S, et al. 2021. Front Immunol. 11:565869. PubMed Slingsby MHL, et al. 2021. Haematologica. Online ahead of print. PubMed Artemenko E, et al. 2016. Biochem J. 473: 435 - 448. PubMed Best MG et al. 2017. Cancer cell. 32(2):238-252 . PubMed Sung PS, et al. 2019. Nat Commun. 10:2402. PubMed Ye Z, et al. 2019. Mediators Inflamm. 2019:8709583. PubMed Petersen-Uribe Á, et al. 2021. Int J Mol Sci. 22:. PubMed Zhang S, et al. 2012. PLoS One. 7:e40641. PubMed Bennett JA, et al. 2020. Nat Commun. 2.874305556. PubMed Lee S, et al. 2021. Nature. 599:283. PubMed Crooks M, et al. 2014. PLoS One. 9:111347. PubMed Welsh J, et al. 2011. Br J Haematol. 154:122. PubMed Marsden AJ, et al. 2021. ACS Pharmacol Transl Sci. 4:1567. PubMed Deng W, et al. 2016. Nat Commun. 7: 12863. PubMed Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed Karampini E, et al. 2019. Haematologica. 104:2091. PubMed\u003cbr\u003e\nRRID: AB_314477 (BioLegend Cat. No. 304905) AB_314478 (BioLegend Cat. No. 304906)\u003cbr\u003e\nStructure: Selectin, type I glycoprotein, 140 kD\u003cbr\u003e\nDistribution: Activated platelets, megakaryocytes, endothelial cells\u003cbr\u003e\nFunction: Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions\u003cbr\u003e\nLigand\/Receptor: CD162 (PSGL-1), CD24 and sialylated Lewis X\u003cbr\u003e\nCell Type: Endothelial cells, Megakaryocytes, Neutrophils, Platelets\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. McEver R, et al. 1995. J. Biol. Chem. 270:11025. 2. Varki A. 1994. P. Natl. Acad. Sci. USA 91:7390.\u003cbr\u003e\nGene ID: 6403\u003cbr\u003e\nUniProt: View information about CD62P on UniProt.org\u003cbr\u003e\nClone: AK4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VI P-44\u003cbr\u003e\nOther Names: GMP-140, PADGEM\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862537654441,"sku":"304905","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-304905","provider":"Iright","version":"1.0","type":"link"}