{"product_id":"biolegend-306502","title":"Biolegend, 306502, Purified anti-human CD184 (CXCR4) Antibody, 100μg","description":"\u003cp\u003eCD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXC chemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and T cells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes\/platelets, lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons, among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates blood cell migration, and is involved in B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and an alternative receptor for some isolates of HIV-2.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Chimpanzee, Sooty Mangabey\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: CP-MAC-infected Sup-T1 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested ICC, CyTOF® - Verified IHC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections11, immunocytochemistry3, immunofluorescence microscopy2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-14,5. Clone 12G5 may not be suitable for Western blotting.10 The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt;0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 306539 \u0026amp; 306540).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): McKnight A, et al. 1997. J. Virol. 71:1692. Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF) Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC) Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block) Ullrich CK, et al. 2000. Blood 96:1438. (Block) Murga M, et al. 2005. Blood 105:1992. (IF) Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed Isnardi I, et al. 2010. Blood 115:5026. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Fischer T, et al. 2008. PLoS One 3:e4069. Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)\u003cbr\u003e\nProduct Citations: Mousset CM, et al. 2018. Oncoimmunology. 7:e1488565. PubMed Thompson B, et al. 2007. J Biol Chem. 282:9547. PubMed Gañán-Gómez I, et al. 2022. Nat Med. . PubMed Kickler K, et al. 2012. J Immunol. 188:5303. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed Kondo N, et al. 2020. Cancers (Basel). 12:00. PubMed Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed\u003cbr\u003e\nRRID: AB_314607 (BioLegend Cat. No. 306501) AB_314608 (BioLegend Cat. No. 306502)\u003cbr\u003e\nStructure: Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD\u003cbr\u003e\nDistribution: T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells\u003cbr\u003e\nFunction: B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development\u003cbr\u003e\nLigand\/Receptor: SDF-1 receptor, coreceptor for X4 HIV-1\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657. 2. Loetscher P, et al. 2000. Adv. Immunol. 74:127. 3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145.\u003cbr\u003e\nGene ID: 7852\u003cbr\u003e\nUniProt: View information about CD184 on UniProt.org\u003cbr\u003e\nClone: 12G5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VII 70204\u003cbr\u003e\nOther Names: CXCR4, Fusin\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860797870249,"sku":"306502","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-306502","provider":"Iright","version":"1.0","type":"link"}