{"product_id":"biolegend-308107","title":"Biolegend, 308107, Alexa Fluor® 488 anti-human Perforin Antibody, 25tests","description":"\u003cp\u003ePerforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Cow\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Purified granules from the human lymphoma cell line\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Clone dG9 primarily recognizes perforin associated with cytotoxic granules9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections1,4.Does not cross-react with mouse1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC) Schaerli P, et al. 2004. J. Exp. Med. 199:1265. Watanabe N, et al. 1997. Blood 90:3662. Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC) Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF) Chen H, et al. 2005. J. Immunol. 175:591. Bryceson YT, et al. 2007. Blood doi:10.1182\/blood-2007-02-074468. (IF) Wood SM, et al. 2009. Blood 114:4117. PubMed Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.\u003cbr\u003e\nProduct Citations: Kennedy PR, et al. 2023. Front Immunol. 14:1060905. PubMed Carisey AF, et al. 2018. Curr Biol. 28:489. PubMed Orange E 2014. Proc Natl Acad Sci U S A. 111:6708. PubMed Kabanova A, et al. 2016. Cell Rep. S2211-1247: 30219-4. PubMed Ambrose AR, et al. 2020. Proc Natl Acad Sci U S A. 117:23717. PubMed Davey M, et al. 2017. Nat Commun. 10.1038\/ncomms14760. PubMed Oyer JL, et al. 2022. Front Immunol. 13:861681. PubMed Salzer E, et al. 2016. Nat Immunol. 17:1352-1360. PubMed Khazen R, et al. 2016. Nat Commun. 7:10823. PubMed\u003cbr\u003e\nRRID: AB_493252 (BioLegend Cat. No. 308108)\u003cbr\u003e\nStructure: 70 kD\u003cbr\u003e\nDistribution: CTL, NK (cytoplasmic granules)\u003cbr\u003e\nFunction: Mediates targeted cell lysis\u003cbr\u003e\nCell Type: NK cells, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361. 2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.\u003cbr\u003e\nGene ID: 5551\u003cbr\u003e\nUniProt: View information about Perforin on UniProt.org\u003cbr\u003e\nClone: dG9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PRF1, P1, PFP, HPLH2\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863008071849,"sku":"308107","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-308107","provider":"Iright","version":"1.0","type":"link"}