{"product_id":"biolegend-308504","title":"Biolegend, 308504, PE anti-human IFN-γ R β chain Antibody, 100tests","description":"\u003cp\u003eIFN-γ R β chain is a 65 kD type I transmembrane protein, also known as accessory factor (AF-1) or IFN-γRII. IFN-γ receptor is composed of an IFN-γ R α chain (CDw119) and a β chain. They are members of the class II cytokine receptor family. The α chain is required for ligand binding and ligand trafficking to the cells and also plays some role in signal transduction. The β chain is critical for delivering IFN-γ mediated pleiotropic signals. IFN-γ R β chain is broadly expressed on a variety of cells at low levels and upregulated on some activated B cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Hamster\u003cbr\u003e\nImmunogen: soluable extracellular domains of the human IFN-γ R β chain\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Western blotting2,3 and immunoprecipitation2,3. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). TThe LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 308506).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Bach EA, et al. 1996. Mol. Cell. Biol. 16:3214. (IP WB) Rosenzweig SD, et al. 2004. J. Immunol. 173:4000. (IP WB) Joseph TD, et al. 2001. J. Biol. Chem. 276:47136. Choi JC, et al. 2007. J. Immunol. 178:1598. Tjon AS, et al. 2014. J Immunol. 192:5625. PubMed\u003cbr\u003e\nProduct Citations: Martínez-Sabadell A, et al. 2022. Cell Rep. 41:111430. PubMed Tjon A, et al. 2014. J Immunol. 192:5625. PubMed Kulling PM, et al. 2018. PLoS One. 13:e0193429. PubMed Ferrando-Martinez S, et al. 2018. J Clin Invest. 128:2089. PubMed Dela Peña–Ponce MG, et al. 2017. Front Pediatr. 5:15. PubMed Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed\u003cbr\u003e\nRRID: AB_314718 (BioLegend Cat. No. 308504)\u003cbr\u003e\nStructure: Ig superfamily, class II cytokine receptor family, associates with CDw119, 65 kD\u003cbr\u003e\nDistribution: All cells, except erythrocytes, at low levels\u003cbr\u003e\nFunction: Transducing signals\u003cbr\u003e\nLigand\/Receptor: IFN-γ\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Basler C, et al. 2002. Int. Rev. Immunol. 21:305. 2. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835.\u003cbr\u003e\nGene ID: 3459\u003cbr\u003e\nUniProt: View information about IFN-gamma R beta chain on UniProt.org\u003cbr\u003e\nClone: 2HUB-159\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VI C-111\u003cbr\u003e\nOther Names: IFN-γRβ, IFNγAF-1\u003cbr\u003e\nIsotype: Hamster IgG\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862331052201,"sku":"308504","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-308504","provider":"Iright","version":"1.0","type":"link"}