{"product_id":"biolegend-308802","title":"Biolegend, 308802, Purified anti-human CD210 (IL-10 R) Antibody, 100μg","description":"\u003cp\u003eCD210, also known as the IL-10 receptor, is a 90-110 kD protein expressed on T cells, B cells, NK cells, monocytes and macrophages. CD210 belongs to the class II cytokine receptor family which includes the IFN-γ receptor (CDw119), the IFN-α\/β receptor (CD118) and tissue factor (CD142). The IL-10 receptor is involved in signal transduction by inducing phosphorylation of STAT1a and STAT3 and by inducing activation of Jak1 and Tyk.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: shIL-10R\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IP, Block - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone 3F9 recognizes the IL-10-binding epitope of IL-10R1.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro blocking1-3 of human IL-10 binding to IL-10R. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-rat IgG second step), followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays (Cat. No. 308817 and 308818).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Liu Y, et al. 1997. J. Immunol. 158:604. (Immunogen, IP, Block) Levings MK, et al. 2005. Blood 105:1162. (Block) Goodier MR, et al. 2000. J. Immunol. 165:139. (Block) Huang YH, et al. 2009. J. Leukoc. Biol. 86:273. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Liu BS,et al. 2011. J Leukoc Biol. 89:981. PubMed Joffe M, et al. 2012. Int Immunol. 24:447. PubMed MacDonald KP, et al. 1999. J. Immunol. 163:5599. (epitope)\u003cbr\u003e\nProduct Citations: Svajger U, et al. 2019. Front Immunol. 2.240972222. PubMed Jenkins BR, et al. 2021. The FASEB Journal. 35(6):e21551. PubMed Ni G, et al. 2019. PLoS One. 14:e0213813. PubMed Huang Y, et al. 2009. J Leukoc Biol. 86:273. PubMed Joffe M, et al. 2012. Int Immunol. 24:447. PubMed Claassen M, et al. 2012. PLoS One. 7:e49389. PubMed Liu B, et al. 2011. J Leukoc Biol. 89:981. PubMed Tang X, et al. 2020. Sci Adv. 6:eaaz0374. PubMed\u003cbr\u003e\nRRID: AB_314734 (BioLegend Cat. No. 308802)\u003cbr\u003e\nStructure: Ig superfamily, class II cytokine receptor family, transmembrane glycoprotein, 90-110 kD\u003cbr\u003e\nDistribution: T cells and B cells, NK cells, monocytes, macrophages\u003cbr\u003e\nFunction: Activates STAT1, STAT3, Jak1, Tyk\u003cbr\u003e\nLigand\/Receptor: IL-10\u003cbr\u003e\nCell Type: B cells, Macrophages, Monocytes, NK cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Kotenko S. 2002. Cytokine Growth Factor Rev. 13:223. 2. Trinchieri G. 2003. Nat. Rev. Immunol. 3:133.\u003cbr\u003e\nGene ID: 3587\u003cbr\u003e\nUniProt: View information about CD210 on UniProt.org\u003cbr\u003e\nClone: 3F9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VII 70502\u003cbr\u003e\nOther Names: IL-10 Receptor, IL-10R\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862396096681,"sku":"308802","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-308802","provider":"Iright","version":"1.0","type":"link"}