{"product_id":"biolegend-309405","title":"Biolegend, 309405, Biotin anti-human CD275 (B7-H2, ICOSL) Antibody, 25μg","description":"\u003cp\u003eB7-H2, a member of the B7 family and the immunoglobulin superfamily, is a 40 kD protein also known as B7RP-1, B7h, B7-H2, GL50 and ICOS Ligand. Human B7-H2 is expressed by B lymphocytes, activated monocytes\/macrophages, and dendritic cells. B7-H2 binds to a CD28-like receptor, inducible costimulator molecule (ICOS, AILIM, CRP-1), which is expressed by activated T cells. The interaction of ICOS with B7-H2 plays an important role in the T cell costimulation pathway.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human B7H2-mIg fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kurosawa S, et al. 2003. Am. J. Respir. Cell Mol. Biol. 28:563.\u003cbr\u003e\nProduct Citations: Pallikkuth S, et al. 2020. eLife. 9:e51889.. PubMed Martín–Gayo E, et al. 2020. Cell Rep. 30:984. PubMed\u003cbr\u003e\nRRID: AB_528729 (BioLegend Cat. No. 309406)\u003cbr\u003e\nStructure: Ig superfamily, B7 family, 40 kD\u003cbr\u003e\nDistribution: B cells and dendritic cells\u003cbr\u003e\nFunction: Binds ICOS on antigen-activated T cells and mediates proliferation and cytokine production\u003cbr\u003e\nLigand\/Receptor: ICOS\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Wang S, et al. 2002. J. Biol. Chem. 96:2808. Wong SC, et al. 2003. Blood 102:1831.\u003cbr\u003e\nGene ID: 23308\u003cbr\u003e\nUniProt: View information about CD275 on UniProt.org\u003cbr\u003e\nClone: 2D3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD275, ICOSL, B7H2, ICOS-L, B7RP-1, B7-RP1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862317125801,"sku":"309405","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-309405","provider":"Iright","version":"1.0","type":"link"}