{"product_id":"biolegend-309804","title":"Biolegend, 309804, PE anti-human CD137 (4-1BB) Antibody, 100tests","description":"\u003cp\u003eCD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Ectodomain of recombinant human 4-1BB fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP) Salih HR, et al. 2000. J. Immunol. 165:2903. (FA) Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA) Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)\u003cbr\u003e\nProduct Citations: Liu T, et al. 2021. Gut. 70:1965. PubMed Chow A 2023. Immunity. 56(1):93-106.e6. PubMed Shitaoka K, et al. 2018. Cancer Immunol Res. 6:378. PubMed Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed Yamaguchi S, et al. 2021. Eur J Immunol. 51:2306. PubMed Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed Goncharov MM, et al. 2022. Elife. 11:. PubMed Shin JJ, et al. 2022. J Clin Immunol. :. PubMed Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed Pavlicek D, et al. 2017. Immun Ageing. 14:22. PubMed Gordon-Alonso M,et al. 2017. Nat. Commun. 10.1038\/s41467-017-00925-6. PubMed Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed Jung JH, et al. 2021. Nat Commun. 12:4043. PubMed Prado-Garcia H, et al. 2017. Cancer Immunol Immunother. 10.1007\/s00262-017-1979-x. PubMed Elias G, et al. 2022. Elife. 11:. PubMed Correnti CE, et al. 2020. Nat Struct Mol Biol. 27:342. PubMed Gao R, et al. 2018. Oncoimmunology. 7:e1438108. PubMed Tian L, et al. 2022. Clin Cancer Res. 28:201. PubMed Senger K, et al. 2022. Curr Protoc. 2:e538. PubMed Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed\u003cbr\u003e\nRRID: AB_314782 (BioLegend Cat. No. 309803) AB_314783 (BioLegend Cat. No. 309804)\u003cbr\u003e\nStructure: TNFR superfamily, type I transmembrane protein, 30 kD\u003cbr\u003e\nDistribution: Activated T cells\u003cbr\u003e\nFunction: T cell costimulation\u003cbr\u003e\nLigand\/Receptor: 4-1BB ligand\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Gruss H, et al. 1995. Blood 85:3378. 2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355. 3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219. 4. Schwarz H, et al. 1996. Blood 87:2839.\u003cbr\u003e\nGene ID: 3604\u003cbr\u003e\nUniProt: View information about CD137 on UniProt.org\u003cbr\u003e\nClone: 4B4-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VI C-7\u003cbr\u003e\nOther Names: 4-1BB, ILA, CD137, TNFRSF9\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862411464873,"sku":"309804","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-309804","provider":"Iright","version":"1.0","type":"link"}