{"product_id":"biolegend-310107","title":"Biolegend, 310107, Purified anti-human CXCL1 (GROα) Antibody, 100μg","description":"\u003cp\u003eCXCL1, also known as GROα, is an ELR+ CXC chemokine that is structurally and functionally related to CXCL2 (GRO2), CXCL3 (GRO3), and CXCL8 (IL-8). CXCL1, CXCL3, and IL-8 bind to the CXCR2 receptor (CD182) with CXCL1 (GROα) having the highest affinity. CXCL1 has pleiotropic effects on cell proliferation, tumor angiogenesis, invasion, and metastasis and is crucial for the recruitment of neutrophils to inflammatory sites.  CXCL1 is frequently overexpressed in many cancers, such as breast cancer, ovarian cancer, colorectal cancer, prostate cancer, and squamous cell carcinoma. Thrombin enhances the secretion of CXCL1 from tumor cells, and these two molecules together induce vascular regulatory proteins and growth factors involved in angiogenesis; this parallels the activity of VEGF, Ang-2, CD31, KDR, CXCR2, MMP1, and MMP2 in HUVEC cells. In addition, PGE2 stimulates expression of CXCL1 in human colorectal carcinoma (CRC) cells through activation of an EGFR-MAPK. CXCL1 (secreted by CRC cells) induces endothelial cell migration and tube formation and is induced by inflammatory cytokines such as IL-1 and TNF. In addition, IL-17A enhances expression of CXCL1 by prolonging the half-life of its constitutively unstable mRNA.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human CXCL1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_3698963 (BioLegend Cat. No. 310107)\u003cbr\u003e\nStructure: Chemokine\u003cbr\u003e\nDistribution: CXCL1 is produced by neutrophils, activated monocytes, fibroblasts, epithelial cells, and endothelial cells\u003cbr\u003e\nFunction: CXCL1 chemoattracts neutrophils and induces cell proliferation, tumor angiogenesis, invasion, and metastasis. CXCL1 is induced by LPS, IL-1, TNF, thrombin, and PGE2.\u003cbr\u003e\nInteraction: Neutrophils and endothelial cells\u003cbr\u003e\nLigand\/Receptor: CXCR2\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: Anisowicz A, et al. 1987. P. Natl. Acad. Sci. USA 84:7188-92. Caunt M, et al. 2006. Cancer Res. 66:4125-32. Wang D, et al. 2006. J Exp Med. 203:941-51. Datta S, et al. 2010. J Immunol. 184:1484-91.\u003cbr\u003e\nGene ID: 2919\u003cbr\u003e\nUniProt: View information about CXCL1 on UniProt.org\u003cbr\u003e\nClone: A19037I\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Fibroblast secretory protein (FSP), Growth regulated oncogene 1 (GRO1), Growth regulated oncogene a (GRO a), Growth-regulated alpha protein, melanoma growth stimulating activity (MGSA), MGSA-α, neutrophil activating protein 3 (NAP-3), SCYB1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866066407593,"sku":"310107","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-310107","provider":"Iright","version":"1.0","type":"link"}