{"product_id":"biolegend-311505","title":"Biolegend, 311505, APC anti-human CD137L (4-1BB Ligand) Antibody, 25tests","description":"\u003cp\u003e4-1BB ligand, also known as CD137L, is a 97 kD member of the TNF superfamily mainly expressed on APCs, activated B and T cells. It has been reported to be important in T cell proliferation and cytokine production through interaction with 4-1BB receptor. 4-1BB ligand appears to be able to act as a costimulatory molecule without the engagement of other costimulatory molecules such as CD28.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 311504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Gullo C, et al. 2010. PLoS One. 5:e10845. (FC) PubMed\u003cbr\u003e\nProduct Citations: Thieme CJ, et al. 2019. Sci Rep. 9:19037. PubMed Lah S, et al. 2023. J Immunother Cancer. 11:. PubMed Vidard L, et al. 2023. Immun Inflamm Dis. 11:e749. PubMed Vidard L, et al. 2019. J Immunol. 203:676. PubMed\u003cbr\u003e\nRRID: AB_2561310 (BioLegend Cat. No. 311505) AB_2561311 (BioLegend Cat. No. 311506)\u003cbr\u003e\nStructure: TNF superfamily, 97 kD\u003cbr\u003e\nDistribution: Antigen presenting cells, activated B cells and T cells\u003cbr\u003e\nFunction: T cell proliferation, cytokine production\u003cbr\u003e\nLigand\/Receptor: 4-1BB (CDw137)\u003cbr\u003e\nCell Type: Antigen-presenting cells, B cells, T cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Akiba H, et al. 2000. J. Exp. Med. 191:375. 2. Pollak KE, et al. 1995. Eur. J. Immunol. 25:488. 3. DeBenedette MA, et al. 1997. J. Immunol. 158:551. 4. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.\u003cbr\u003e\nGene ID: 8744\u003cbr\u003e\nUniProt: View information about CD137L on UniProt.org\u003cbr\u003e\nClone: 5F4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: 4-1BBL, CD137L, TNFSF9, 41BBL\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862461337769,"sku":"311505","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-311505","provider":"Iright","version":"1.0","type":"link"}