{"product_id":"biolegend-313504","title":"Biolegend, 313504, Biotin anti-human\/mouse\/rat CD278 (ICOS) Antibody, 500μg","description":"\u003cp\u003eICOS, also known as inducible costimulatory molecule and H4, is a 47-57 kD protein. This protein is homologous to the CD28\/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h\/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus, Pig\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Mouse T cell clone D10.G4.1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that reagents be titrated for optimal performance in the particular application.\u003cbr\u003e\nApplication Notes: The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim) Yagi J, et al. 2003. J. Immunol. 171:783. (FC) Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim) Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Mouslim C, et al. 2012. J Neurosci. 5177:32. PubMed Lobato-Pascual A, et al. 2013. PLoS One. e57406:8. PubMed\u003cbr\u003e\nRRID: AB_416327 (BioLegend Cat. No. 313503) AB_416328 (BioLegend Cat. No. 313504)\u003cbr\u003e\nStructure: CD28\/CTLA-4, 47-57 kD\u003cbr\u003e\nDistribution: Activated T cells, subset of thymocytes\u003cbr\u003e\nFunction: Costimulates T cell activation, proliferation, humoral immune response\u003cbr\u003e\nLigand\/Receptor: B7h\/B7RP-1\/GL-50\u003cbr\u003e\nCell Type: T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. 2. Hutloff A, et al. 1999. Nature 397:263. 3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463. 4. Coyle AJ, et al. 2000. Immunity 13:95.\u003cbr\u003e\nGene ID: 1000488412985164545\u003cbr\u003e\nUniProt: View information about CD278 on UniProt.org\u003cbr\u003e\nClone: C398.4A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Inducible COStimulatory molecule, H4\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862942666921,"sku":"313504","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-313504","provider":"Iright","version":"1.0","type":"link"}