{"product_id":"biolegend-313510","title":"Biolegend, 313510, APC anti-human\/mouse\/rat CD278 (ICOS) Antibody, 100μg","description":"\u003cp\u003eICOS, also known as inducible costimulatory molecule and H4, is a 47-57 kD protein. This protein is homologous to the CD28\/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS is involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7h\/B7RP-1 or B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus, Pig\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Mouse T cell clone D10.G4.1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that reagents be titrated for optimal performance in the particular application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The C398.4A antibody is useful for flow cytometric analysis and is able to costimulate T cell activation and proliferation. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T cell activation1,3,4, and spatial biology (IBEX)5,6. The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 313512).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. (FC IP Costim) Yagi J, et al. 2003. J. Immunol. 171:783. (FC) Arimura Y, et al. 2002. Int. Immunol. 14:555. (Costim) Arimura Y, et al. 2004. J. Biol. Chem. 279:11408. (Costim) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Woodcock V, et al. 2023. BJUI Compass. 4:322. PubMed Wu X, et al. 2023. iScience. 26:106559. PubMed Peng C, et al. 2022. Immunity. 55:98. PubMed Rodda LB, et al. 2022. Cell. 185:1588. PubMed Wijesinghe A, et al. 2020. J Biomed Sci. 27:50:00. PubMed Arce Vargas F et al. 2018. Cancer cell. 33(4):649-663 . PubMed Karim F, et al. 2021. Elife. 10:. PubMed Miles B, et al. 2015. Nat Commun. 6: 8608. PubMed Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed Court AC, et al. 2020. EMBO Rep. 21:e48052. PubMed Zhang Y, et al. 2021. J Immunol Res. 2021:5599439. PubMed Cassotta A, et al. 2020. J Exp Med. 217:00:00. PubMed Sefik E, et al. 2021. Nat Biotechnol. . PubMed Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed Zhang SL, et al. 2022. Gut Microbes. 14:2046246. PubMed Bonnet B, et al. 2016. J Immunol. 197: 188 - 198. PubMed Paul S, et al. 2021. Sci Transl Med. 13:. PubMed Llibre A, et al. 2016. J Immunol. 196: 2085 - 2094. PubMed NULL, et al. 2022. Cell. 185:916. PubMed Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed Lim S, et al. 2017. Sci Rep. 7:40040. PubMed Deakin A, et al. 2014. PLoS One. 9:107490. PubMed Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed Cassotta A, et al. 2020. Eur J Immunol. . PubMed\u003cbr\u003e\nRRID: AB_416333 (BioLegend Cat. No. 313509) AB_416334 (BioLegend Cat. No. 313510)\u003cbr\u003e\nStructure: CD28\/CTLA-4, 47-57 kD\u003cbr\u003e\nDistribution: Activated T cells, subset of thymocytes\u003cbr\u003e\nFunction: Costimulates T cell activation, proliferation, humoral immune response\u003cbr\u003e\nLigand\/Receptor: B7h\/B7RP-1\/GL-50\u003cbr\u003e\nCell Type: T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Redoglia V, et al. 1996. Eur. J. Immunol. 26:2781. 2. Hutloff A, et al. 1999. Nature 397:263. 3. Buonfiglio D, et al. 2000. Eur. J. Immunol. 30:3463. 4. Coyle AJ, et al. 2000. Immunity 13:95.\u003cbr\u003e\nGene ID: 1000488412985164545\u003cbr\u003e\nUniProt: View information about CD278 on UniProt.org\u003cbr\u003e\nClone: C398.4A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Inducible COStimulatory molecule, H4\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862943322281,"sku":"313510","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-313510","provider":"Iright","version":"1.0","type":"link"}