{"product_id":"biolegend-315807","title":"Biolegend, 315807, APC anti-human CD177 Antibody, 25tests","description":"\u003cp\u003eCD177 is also known as neutrophil specific antigen 1, NB1, and polycythemia rubra vera 1. It is a member of the uPAR family and is a GPI-linked cell surface glycoprotein with a molecular weight of 60 kD. CD177 is expressed on granulocytes and bone marrow progenitors (early erythroblasts, megakaryocytes). It is thought to be involved in allogeneic and autoimmune responses to neutrophils.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human granulocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting5, and immunofluorescence4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Leucocyte Typing VII. Mason D, et al. Eds, 2002 Oxford University Press. von Vietinghoff S, et al. 2007. Blood 109:4487. PubMed Korkmaz B, et al. 2008. J. Biol. Chem. 283:35976. PubMed von Vietinghoff S, et al. 2007. Blood 109:4487. (IF) Jankowska AM, et al. 2011. Haematologica. 96:954. (WB)\u003cbr\u003e\nProduct Citations: Mettelman RC, et al. 2023. Nat Immunol. 1511:24. PubMed\u003cbr\u003e\nRRID: AB_2565213 (BioLegend Cat. No. 315807) AB_2565214 (BioLegend Cat. No. 315808)\u003cbr\u003e\nStructure: uPAR family, GPI-linked cell surface glycoprotein, 60 kD\u003cbr\u003e\nDistribution: Granulocytes, bone marrow progenitors (early erythroblasts, megakaryocytes)\u003cbr\u003e\nFunction: Antigen involved in neutrophil allo- and autoimmunity, function unknown\u003cbr\u003e\nModification: Glycosylated\u003cbr\u003e\nCell Type: Granulocytes, Hematopoietic stem and progenitors, Neutrophils\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Leukocyte Typing VII. Mason D, et al. (Eds.) Oxford University Press (2002) 2. Kissel K, et al. 2001. Eur. J. Immunol. 31:1301. 3. Lalezari P, et al. 1971. J. Clin. Invest. 50:1108. 4. Temerinac S, et al. 2000. Blood 95:2569.\u003cbr\u003e\nGene ID: 57126\u003cbr\u003e\nUniProt: View information about CD177 on UniProt.org\u003cbr\u003e\nClone: MEM-166\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: Neutrophil specific antigen 1, NB1, polycythemia rubra vera 1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866462769321,"sku":"315807","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-315807","provider":"Iright","version":"1.0","type":"link"}