{"product_id":"biolegend-316202","title":"Biolegend, 316202, Purified anti-human CD72 Antibody, 100μg","description":"\u003cp\u003eCD72 is a 39-43 kD type II membrane glycoprotein. It is a disulfide-linked homodimer belonging to C-type lectin family. CD72 is a pan-B cell marker expressed on pre-pre-B cells throughout B cell differentiation with the exception of plasma cells. It is also expressed on follicular dendritic cells, splenic red pulp macrophages (but not on peripheral blood monocytes), and liver Kupffer cells. CD72, a negative coreceptor of B cells, contains immunoreceptor tyrosine-based inhibitory motifs in the cytoplasmic domain which has been shown to recruit the tyrosine phosphatase SHP-1. Ligation of CD72 with its ligand regulates CD72 tyrosine dephosphorylation and SHP-1 dissociation to promote B cell activation and proliferation. CD100 and CD5 have been shown to be CD72 ligands. The CD100-CD72 interaction plays a role in maintenance of B cell homeostasis.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human lymphocytes\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.\u003cbr\u003e\nProduct Citations: Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed\u003cbr\u003e\nRRID: AB_2260104 (BioLegend Cat. No. 316202)\u003cbr\u003e\nStructure: Type II membrane glycoprotein, C-type lectin family, 39-43 kD, homodimer\u003cbr\u003e\nDistribution: Pre-pre-B cells throughout B cells differentiation (but not on plasma cells), follicular dendritic cells, splenic red pulp macrophages, liver Kupffer cells\u003cbr\u003e\nFunction: negative regulation of B cell receptor signaling\u003cbr\u003e\nLigand\/Receptor: CD5, CD100\/Sema4D\u003cbr\u003e\nCell Type: B cells, Dendritic cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. Schwarting T, et al. 1992. Am. J. Hematol. 41:151. Wu HJ and S. Bondata. 2002. Immunol. Res. 25:155. Kumanogoh A, et al. 2000. Immunity 13:621. Parnes JR and C. Pan. 2000. Immunol. Rev. 176:75. Kumanogoh A, et al. 2005. Intnl. Immunol. 17:1277.\u003cbr\u003e\nGene ID: 971\u003cbr\u003e\nUniProt: View information about CD72 on UniProt.org\u003cbr\u003e\nClone: 3F3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VI CD72.1\u003cbr\u003e\nOther Names: Lyb-2, Ly-19.2, Ly-32.2\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866450219177,"sku":"316202","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-316202","provider":"Iright","version":"1.0","type":"link"}