{"product_id":"biolegend-317336","title":"Biolegend, 317336, PerCP\/Cyanine5.5 anti-human CD3 Antibody, 100tests","description":"\u003cp\u003eCD3ε is a 20 kD chain of the CD3\/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α\/β or γ\/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt;0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. Li B, et al. 2005. Immunology 116:487. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed Alter G, et al. 2008. J. Virol. 82:9668. PubMed Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed Pinto JP, et al. 2010. Immunology. 130:217. PubMed Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed\u003cbr\u003e\nProduct Citations: Beck C, et al. 2023. Biomedicines. 11:. PubMed Kaku CI, et al. 2023. Nat Commun. 14:2751. PubMed Aschmoneit N, et al. 2021. J Immunother Cancer. 9:. PubMed Eiva MA, et al. 2022. Eur J Immunol. 52:96. PubMed Barnes CO, et al. 2022. Sci Adv. 8:eabp8155. PubMed Khuisangeam N, et al. 2022. J Immunol Res. 2022:2449373. PubMed Lee E, et al. 2021. Sci Rep. 11:14841. PubMed Sim J, et al. 2019. MAbs. 11:1036. PubMed Kasper M, et al. 2021. Elife. 10:. PubMed Wang J, et al. 2020. Cell. 183:1264. PubMed Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed Narsale A, Moya R, Robertson H 2016. Data Brief. 8: 1348-51. PubMed Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed Billing F, et al. 2021. ACS Appl Mater Interfaces. 13:55534. PubMed Zhang Z, et al. 2021. Acta Pharm Sin B. 11:1965. PubMed Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed Bulgarelli J, et al. 2021. Front Immunol. 12:778459. PubMed Aschmoneit N, et al. 2022. Oncoimmunology. 11:2028961. PubMed Wu X, et al. 2021. Front Immunol. 12:731767. PubMed Aschmoneit N, et al. 2021. Sci Rep. 11:13880. PubMed Schoofs T et al. 2019. Immunity. 50(6):1513-1529 . PubMed Mudd PA, et al. 2022. Cell. 185:603. PubMed Alonso R, et al. 2022. Blood Adv. :. PubMed Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed Collinson–Pautz MR, et al. 2019. Leukemia. 33:2195. PubMed Wu J, et al. 2021. Adv Sci (Weinh). 8:e2101029. PubMed Albayati Z, et al. 2017. Scand J Immunol. 86:396. PubMed Ott M, et al. 2020. JCI Insight. 5(17):134386. PubMed\u003cbr\u003e\nRRID: AB_2561627 (BioLegend Cat. No. 317335) AB_2561628 (BioLegend Cat. No. 317336)\u003cbr\u003e\nStructure: Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α\/β or γ\/δ) form the CD3\/TCR complex, 20 kD\u003cbr\u003e\nDistribution: Mature T and NK T cells, thymocyte differentiation\u003cbr\u003e\nFunction: Antigen recognition, signal transduction, T cell activation\u003cbr\u003e\nLigand\/Receptor: Peptide antigen bound to MHC\u003cbr\u003e\nCell Type: NKT cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. Lanier L, et al. 1986. J. Immunol. 137:2501.\u003cbr\u003e\nGene ID: 916\u003cbr\u003e\nUniProt: View information about CD3 on UniProt.org\u003cbr\u003e\nClone: OKT3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: T3, CD3ε\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866158223529,"sku":"317336","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-317336","provider":"Iright","version":"1.0","type":"link"}