{"product_id":"biolegend-317424","title":"Biolegend, 317424, Pacific Blue™ anti-human CD4 Antibody, 100μg","description":"\u003cp\u003eCD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes\/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: Chimpanzee\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human peripheral T cells\u003cbr\u003e\nFormulation: test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.\u003cbr\u003e\nConcentration: µg sizes: 0.5 mg\/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For test size, the suggested use of this reagent for immunofluorescent staining is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For µg sizes, the suggested use of this reagent for immunofluorescent staining is ≤2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454). In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36. Linder J, et al. 1987. Am. J. Pathol. 127:1. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC) Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen) Lederman S, et al. 1991. Mol Immunol. 28:1171-81.\u003cbr\u003e\nProduct Citations: XP H, et al. 2015. Nat Commun. 6: 8020. PubMed Tew G, et al. 2016. Gastroenterology. 150:477-487. PubMed Metelo AM, et al. 2022. Cancer Res Commun. 2:158. PubMed Wijesinghe A, et al. 2020. J Biomed Sci. 27:50:00. PubMed Zhu W, et al. 2021. Onco Targets Ther. 14:2149. PubMed Causi E, et al. 2015. PLoS One. 10: 0136717. PubMed Estes J, et al. 2015. J Infect Dis. 211:744. PubMed Wang J, et al. 2020. Cell. 183:1264. PubMed Eckard AR, et al. 2017. Antivir Ther. 1.177083333. PubMed McGary C, et al. 2014. J Virol. 88:4533. PubMed Wu G, et al. 2021. Front Microbiol. 12:636703. PubMed Eckard AR, et al. 2017. Antivir Ther. 1.38125. PubMed Theobald SJ, et al. 2022. EMBO Mol Med. 14:e15888. PubMed Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed Fang F, et al. 2021. Cell Rep. 37:109981. PubMed Ji C, et al. 2009. J Biol Chem. 284:5175. PubMed Hasenkrug KJ, et al. 2021. MBio. 12:e0150321. PubMed Duque JSR, et al. 2022. Nat Commun. 13:3700. PubMed Tabb B, et al. 2012. J Infect Dis. 207:880. PubMed Robert R, et al. 2017. PLoS One. 10.1371\/journal.pone.0184278. PubMed Rnjak D, et al. 2021. Transfus Clin Biol. . PubMed Xie CB, et al. 2020. J Clin Invest. 130:3437. PubMed Chowdhury A, et al. 2015. J Virol. 89: 8677-8686. PubMed Wang S, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed\u003cbr\u003e\nRRID: AB_571952 (BioLegend Cat. No. 317423) AB_1595438 (BioLegend Cat. No. 317429) AB_571953 (BioLegend Cat. No. 317424)\u003cbr\u003e\nStructure: Ig superfamily, type I transmembrane glycoprotein, 55 kD\u003cbr\u003e\nDistribution: T cell subset, majority of thymocytes, monocytes\/macrophages\u003cbr\u003e\nFunction: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor\u003cbr\u003e\nLigand\/Receptor: MHC class II molecules, HIV gp120, IL-16\u003cbr\u003e\nCell Type: Macrophages, Monocytes, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Center D, et al. 1996. Immunol. Today 17:476. 2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.\u003cbr\u003e\nGene ID: 920\u003cbr\u003e\nUniProt: View information about CD4 on UniProt.org\u003cbr\u003e\nClone: OKT4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: T4\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: I am unable to see expression of T cell markers such as CD3 and CD4 post activation.\u003cbr\u003e\nA: TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA\/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866159796393,"sku":"317424","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-317424","provider":"Iright","version":"1.0","type":"link"}