{"product_id":"biolegend-317425","title":"Biolegend, 317425, Alexa Fluor® 700 anti-human CD4 Antibody, 25μg","description":"\u003cp\u003eCD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes\/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: Chimpanzee\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human peripheral T cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm \/ 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454). In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36. Linder J, et al. 1987. Am. J. Pathol. 127:1. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC) Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen) Lederman S, et al. 1991. Mol Immunol. 28:1171-81.\u003cbr\u003e\nProduct Citations: Varun K, et al. 2023. EBioMedicine. 90:104516. PubMed Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed Paniskaki K, et al. 2022. Transplant Proc. 54:1455. PubMed Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed Balta E, et al. 2023. Front Immunol. 13:1063313. PubMed Lovelace SE, et al. 2022. iScience. 25:105067. PubMed Gordon E, et al. 2016. Proc Natl Acad Sci U S A. 113: 8765 - 8770. PubMed Cerosaletti K, et al. 2017. The Journal of Immunology. 10.4049\/jimmunol.1700172. PubMed Ott PA, et al. 2017. Nature. 547:217. PubMed Naaber P, et al. 2022. Cell Rep Med. 3:100716. PubMed Asokan M, et al. 2020. Proc Natl Acad Sci U S A. 117:18754. PubMed Aschauer C, et al. 2021. Front Immunol. 12:750005. PubMed Sabree SA, et al. 2021. J Immunother Cancer. 9: . PubMed Frencher J, et al. 2014. J Leukoc Biol. 96:957. PubMed Thieme CJ, et al. 2020. Cell Rep Med. 1:100092. PubMed Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed Salumets A, et al. 2022. Aging Cell. 21:e13607. PubMed\u003cbr\u003e\nRRID: AB_571942 (BioLegend Cat. No. 317425) AB_571943 (BioLegend Cat. No. 317426)\u003cbr\u003e\nStructure: Ig superfamily, type I transmembrane glycoprotein, 55 kD\u003cbr\u003e\nDistribution: T cell subset, majority of thymocytes, monocytes\/macrophages\u003cbr\u003e\nFunction: MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor\u003cbr\u003e\nLigand\/Receptor: MHC class II molecules, HIV gp120, IL-16\u003cbr\u003e\nCell Type: Macrophages, Monocytes, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Center D, et al. 1996. Immunol. Today 17:476. 2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.\u003cbr\u003e\nGene ID: 920\u003cbr\u003e\nUniProt: View information about CD4 on UniProt.org\u003cbr\u003e\nClone: OKT4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: T4\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: I am unable to see expression of T cell markers such as CD3 and CD4 post activation.\u003cbr\u003e\nA: TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA\/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866159599785,"sku":"317425","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-317425","provider":"Iright","version":"1.0","type":"link"}