{"product_id":"biolegend-318807","title":"Biolegend, 318807, APC anti-human CD270 (HVEM, TR2) Antibody, 25tests","description":"\u003cp\u003eThe 122 antibody recognizes human HVEM also known as herpesvirus entry mediator A, tumor necrosis factor receptor superfamily, member 14, TNFRSF14, and tumor necrosis factor receptor like 2. HVEM, a member of the TNFR superfamily, is a type I transmembrane protein containing 2 TNF receptor domains with a predicted molecular weight of approximately 30 kD. HVEM is widely expressed in blood vessels, brain, heart, kidney, liver, lung, prostate, spleen, thymus and other organs. Resting T cells and naïve and memory B cells express high levels of HVEM as well. In humans, HVEM is not expressed in germinal center B cells. Immature dendritic cells express high levels of HVEM that is downregulated upon maturation. HVEM plays an important role in herpes simplex virus pathogenesis by enhancing entry into cells. Signaling through HVEM activates JNK1, NF-κB and AP-1 to control gene expression in response to infection or cellular stress and activate the immune response. HVEM binds to LIGHT and has also been shown to associate with several other proteins including TRAF1, TRAF2, TRAF3, TRAF5, B and T lymphocyte associated protein (BTLA), and estrogen receptor alpha.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human HVEM protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The 122 antibody has been shown to be useful for flow cytometry, Western blot, and ELISA.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Cheung TC, et al. 2010. J. Immunol. 185:1949. PubMed Hobo W, et al. 2012. J Immunol. 189:39. PubMed.\u003cbr\u003e\nProduct Citations: Sun W, et al. 2023. Nature. 614:309. PubMed Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed Manresa MC, et al. 2020. Gastroenterology. 159:1778. PubMed\u003cbr\u003e\nRRID: AB_2565252 (BioLegend Cat. No. 318807) AB_2565253 (BioLegend Cat. No. 318808)\u003cbr\u003e\nStructure: Member of the TNFR superfamily, type I transmembrane protein containing 2 TNF receptor domains. Predicted molecular weight approximately 30 kD.\u003cbr\u003e\nDistribution: Widely expressed in blood vessels, brain, heart, kidney, liver, lung, prostate, spleen, thymus and other organs. Resting T cells and naïve and memory B cells express high levels of HVEM. Immature dendritic cells express high levels of HVEM that is downregulated upon maturation.\u003cbr\u003e\nFunction: Plays an important role in herpes simplex virus pathogenesis by enhancing entry into cells. Signaling through HVEM activates JNK1, NF-κB and AP-1 to control gene expression in response to infection or cellular stress and activate the immune response.\u003cbr\u003e\nInteraction: TRAF1, TRAF2, TRAF3, TRAF5, B and T lymphocyte associated protein (BTLA), and estrogen receptor alpha have been shown to directly interact with HVEM in vivo.\u003cbr\u003e\nLigand\/Receptor: LIGHT (TNFSF14), LTα\u003cbr\u003e\nCell Type: B cells, Dendritic cells, T cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Carfi A, et al. 2001. Molec. Cell 8:169. 2. Gonzalez LC, et al. 2005.Proc. Nat. Acad. Sci. 102:1116. 3. Kwon BS, et al. 1997. J. Biol. Chem. 272:13471. 4. Marsters SA, et al. 1997. J. Biol. Chem. 272:14272. 5. Montgomery RI, et al. 1996. Cell 87:427.\u003cbr\u003e\nGene ID: 8764\u003cbr\u003e\nUniProt: View information about CD270 on UniProt.org\u003cbr\u003e\nClone: 122\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: TR2, Herpesvirus entry mediator A, Tumor necrosis factor receptor superfamily, member 14, TNFRSF14, Tumor necrosis factor receptor like 2, HVEM\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860794167465,"sku":"318807","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-318807","provider":"Iright","version":"1.0","type":"link"}