{"product_id":"biolegend-320902","title":"Biolegend, 320902, Purified anti-human MICA\/MICB Antibody, 100μg","description":"\u003cp\u003e6D4 antibody reacts with a common epitope of the human nonclassical MHC class I chain-related protein A (MICA) and B (MICB), also known as PERB11.1 and PERB11.2. The MIC gene is located in MHC class I region. MICA\/B are 65-75 kD stress-inducible glycoproteins with highly polymorphic. They are MHC class I-like transmembrane molecules that do not associate β2-microglobulin and do not present peptides. MICA and MICB share 85% identify, and are mainly expressed on Intestinal epithelial cells, epithelial tumor cells, endothelial cells, fibroblasts, and IFN-α-stimulated dendritic cells. MIC molecules bind NKG2D, an activating receptor, and induce activation of NK cells, and subset of CD8 + α\/β T cells and γ\/δ T cells, as well as suppression of T cell proliferation. MICA\/B recognition is involved in the regulation of tumor surveillance, viral infection and autoimmune diseases. The 6D4 antibody is able to block MIC mediated cytotoxicity.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC-P, IHC-F, IP, Block - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported (for the relevant formats) applications include: immunohistochemistry2,3,5 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation7, and blocking2,3 of MIC mediated cytotoxicity. The Ultra-LEAF™ Purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 320919 \u0026amp; 320120).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Groh V, et al. 1999. Science 279:1737. Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879. Groh V, et al. 2001. Nature Immunol. 2:255. Li Z, et al. 2000. Immunogenetics 51:246. Park EJ, et al. 2003. J. Immunol. 171:4131. Jinushi M, et al. 2003. J. Immunol. 171:5423. Wu J, et al. 2003. J. Immunol. 170:4196.\u003cbr\u003e\nProduct Citations: Nakajima NI, et al. 2017. Oncol Rep. 38:693. PubMed Nolan E, et al. 2017. Sci Transl Med. 9:eaal4925. PubMed Tjwa E, et al. 2012. J Virol. 86:4102. PubMed Wang J, et al. 2022. Oncoimmunology. 11:2016159. PubMed Takahara M, et al. 2008. J Leukoc Biol. 83:742. PubMed\u003cbr\u003e\nRRID: AB_493189 (BioLegend Cat. No. 320902)\u003cbr\u003e\nStructure: 65-75 kD glycoprotein, MHC class I-like transmembrane molecules that do not associate ß2-microglobulin, highly polymorphic\u003cbr\u003e\nDistribution: Intestinal epithelium, epithelial tumors, endothelial cells, fibroblasts, INF-a-stimulated dendritic cells\u003cbr\u003e\nFunction: Activation of NK cells, subset of CD8+ α\/ß T cells and γ\/δ T cells, suppression of T cell proliferation\u003cbr\u003e\nLigand\/Receptor: NKG2D\u003cbr\u003e\nCell Type: Dendritic cells, Endothelial cells, Epithelial cells, Fibroblasts\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Groh V, et al. 1996. Proc. Natl. Acad. Sci. USA. 93:12445. 2. Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879. 3. Jinushi M, et al. 2003. J. Immunol. 170:1249. 4. Kriegeskorte AK, et al. 2005. Proc. Natl. Acad. Sci. USA. 102:11805. 5. Groh V, et al. 1999. Science 279:1737.\u003cbr\u003e\nGene ID: 1005074364277\u003cbr\u003e\nUniProt: View information about MICA MICB on UniProt.org\u003cbr\u003e\nClone: 6D4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PERB11, MIC, MHC class I chain-related protein A, MICA, PERB11.1, MHC class I chain-related protein B, MICB, PERB11.2\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862475526313,"sku":"320902","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-320902","provider":"Iright","version":"1.0","type":"link"}