{"product_id":"biolegend-321222","title":"Biolegend, 321222, Alexa Fluor® 647 anti-human\/mouse Integrin β7 Antibody, 100tests","description":"\u003cp\u003eIntegrin β7 is a 130 kD glycoprotein also known as integin βp. It is a member of the Ig superfamily. In association with integrin α4 or αE chain, β7 forms α4\/β7 or αE\/β7 heterodimer. α4\/β7 (CD49d\/β7, LPAM-1) is expressed on the majority of peripheral lymphocytes, on small subsets of thymocytes, and bone marrow progenitors. LPAM-1 binds to several ligands, VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion and some hematopoietic progenitor cells migration. αE\/β7 (CD103\/β7,α IEL \/β7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103\/β7 complex is thought to play a role in lymphocyte retention via interaction with its ligand E-Cadherin.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Chimpanzee\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: TK1 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The FIB504 antibody has been reported to react with mouse and human ß7 integrin and to block ß7 integrin-mediated cell adhesion in in vitro and in vivo studies. Additional reported applications (for the relevant formats) include: blocking of cell adhesion1,3,4. The Ultra-LEAF™ purified FIB504 antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321233-321238).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block) Berlin C, et al. 1993. Cell 74:185. Rott LS, et al. 1996. J. Immunol. 156:3727. (Block) Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block) Ohmori K, et al. 2009. J. Immunol. 182:2835. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)\u003cbr\u003e\nRRID: AB_2715978 (BioLegend Cat. No. 321221) AB_2715979 (BioLegend Cat. No. 321222)\u003cbr\u003e\nStructure: Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103\u003cbr\u003e\nDistribution: CD49d\/β7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors; CD103\/β7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes\u003cbr\u003e\nFunction: Lymphocyte adhesion, hematopoietic progenitor cells migration\u003cbr\u003e\nLigand\/Receptor: CD49d\/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103\/β7: E-cadherin\u003cbr\u003e\nCell Type: Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Andrew DP, et al. 1994. J. Immunol. 153:3847. 2. Picarella D, et al. 1997. J. Immunol. 158:2099. 3. Lefrancois L, et al. 1994. Eur. J. Immunol. 24:635 4. Cepek KL, et al. 1994. Nautre 372:190.\u003cbr\u003e\nGene ID: 164213695\u003cbr\u003e\nUniProt: View information about Integrin beta7 on UniProt.org\u003cbr\u003e\nClone: FIB504\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VI 6T-101, VI A024\u003cbr\u003e\nOther Names: β7 Integrin, integrin βp, ITGB7\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864005693609,"sku":"321222","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-321222","provider":"Iright","version":"1.0","type":"link"}