{"product_id":"biolegend-321244","title":"Biolegend, 321244, Brilliant Violet 785™ anti-human\/mouse integrin β7 Antibody, 100tests","description":"\u003cp\u003eIntegrin β7 is a 130 kD glycoprotein also known as integin βp. It is a member of the Ig superfamily. In association with integrin α4 or αE chain, β7 forms α4\/β7 or αE\/β7 heterodimer. α4\/β7 (CD49d\/β7, LPAM-1) is expressed on the majority of peripheral lymphocytes, on small subsets of thymocytes, and bone marrow progenitors. LPAM-1 binds to several ligands, VCAM-1, MAdCAM-1 and fibronectin, and is involved in lymphocyte adhesion and some hematopoietic progenitor cells migration. αE\/β7 (CD103\/β7,α IEL \/β7) is expressed on intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, T regulatory cells, a subset of CD8+ T cells in lymph nodes and lamina propria. CD103\/β7 complex is thought to play a role in lymphocyte retention via interaction with its ligand E-Cadherin.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Chimpanzee\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: TK1 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780\/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: The FIB504 antibody has been reported to react with mouse and human ß7 integrin and to block ß7 integrin-mediated cell adhesion in in vitro and in vivo studies. Additional reported applications (for the relevant formats) include: blocking of cell adhesion1,3,4. The Ultra-LEAF™ purified FIB504 antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321233-321238).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block) Berlin C, et al. 1993. Cell 74:185. Rott LS, et al. 1996. J. Immunol. 156:3727. (Block) Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block) Ohmori K, et al. 2009. J. Immunol. 182:2835. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)\u003cbr\u003e\nRRID: AB_3105992 (BioLegend Cat. No. 321243) AB_3105992 (BioLegend Cat. No. 321244)\u003cbr\u003e\nStructure: Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103\u003cbr\u003e\nDistribution: CD49d\/β7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors; CD103\/β7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes\u003cbr\u003e\nFunction: Lymphocyte adhesion, hematopoietic progenitor cells migration\u003cbr\u003e\nLigand\/Receptor: CD49d\/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103\/β7: E-cadherin\u003cbr\u003e\nCell Type: Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Andrew DP, et al. 1994. J. Immunol. 153:3847. 2. Picarella D, et al. 1997. J. Immunol. 158:2099. 3. Lefrancois L, et al. 1994. Eur. J. Immunol. 24:635 4. Cepek KL, et al. 1994. Nautre 372:190.\u003cbr\u003e\nGene ID: 164213695\u003cbr\u003e\nUniProt: View information about Integrin beta7 on UniProt.org\u003cbr\u003e\nClone: FIB504\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: VI 6T-101, VI A024\u003cbr\u003e\nOther Names: β7 Integrin, integrin βp, ITGB7\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864008741033,"sku":"321244","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-321244","provider":"Iright","version":"1.0","type":"link"}