{"product_id":"biolegend-325502","title":"Biolegend, 325502, Purified anti-human IgE Antibody, 100μg","description":"\u003cp\u003eIgE is the seventh immunoglobulin made by B cells in the immune response.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human Ig cocktail\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested ELISA, IHC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The MHE-18 antibody reacts with both soluble and membrane human immunoglobulin E (IgE). It does not react with other Ig isotypes. Some formats of the MHE-18 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed.\u003cbr\u003e\nProduct Citations: Balemans D,et al. 2017. Sci Rep. . 10.1038\/s41598-017-12618-7. PubMed Clark D, et al. 2013. J Immunol Methods. 22:1759. PubMed Maehara T, et al. 2019. Medicine (Baltimore). 98:e18300. PubMed Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Cildag S, et al. 2021. Med Pharm Rep. 94:53. PubMed\u003cbr\u003e\nRRID: AB_830847 (BioLegend Cat. No. 325502)\u003cbr\u003e\nDistribution: B cells\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nGene ID: 3497\u003cbr\u003e\nUniProt: View information about IgE on UniProt.org\u003cbr\u003e\nClone: MHE-18\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin E\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866128339113,"sku":"325502","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-325502","provider":"Iright","version":"1.0","type":"link"}