{"product_id":"biolegend-325702","title":"Biolegend, 325702, Purified anti-human CD53 Recombinant Antibody, 100μg","description":"\u003cp\u003eCD53 is a 35-42 kD type III tetraspan membrane protein. It is expressed on all leukocytes, but not on platelets or erythrocytes. CD53 is thought to be involved in signal transduction. It has been shown to interact with a number of proteins including IL-4, CD20, CD2, CD9, IL-2, VLA-4, and CD4. Cross-linking of CD53 promotes B cell activation. The CD53 antigen is highly glycosylated and treatment with endoglycosidase F reduces the apparent molecular weight of this protein by approximately 25 kD. The HI29 antibody has been shown to be useful for flow cytometry, and immunohistochemistry (frozen).\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Recombinant\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2892390 (BioLegend Cat. No. 325702)\u003cbr\u003e\nStructure: Cell surface protein, tetraspan family, four membrane spanning hydrophobic domains, 35-42 kD.\u003cbr\u003e\nDistribution: Hematopoietic cells including neutrophils, monocytes, B cells, T cells (single positive thymocytes and peripheral T cells), and eosinophils. Not expressed on platelets, erythrocytes, and non-hematopoietic cells.\u003cbr\u003e\nFunction: Signal transduction, cross-linking on B cells causes activation.\u003cbr\u003e\nInteraction: IL-4, CD20, CD2, CD9, IL-2, integrin α4, integrin β1, CD4\u003cbr\u003e\nLigand\/Receptor: NK cell, B cells, Neutrophils, Eosinophils\u003cbr\u003e\nAntigen References: Amiot M. 1990. J Immunol. 145:4322-5. Angelisova P, et al. 1990. Immunogenetics. 32:281-5. Olweus J, et al. 1993. J Immunol. 153:4997. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford: Oxford University Press.\u003cbr\u003e\nGene ID: 963\u003cbr\u003e\nUniProt: View information about CD53 on UniProt.org\u003cbr\u003e\nClone: QA19A07\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: TSPAN25\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866184634537,"sku":"325702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-325702","provider":"Iright","version":"1.0","type":"link"}