{"product_id":"biolegend-330105","title":"Biolegend, 330105, PE anti-human CD209 (DC-SIGN) Antibody, 25tests","description":"\u003cp\u003eCD209, known as Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin (DC-SIGN), is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. CD209 is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. Importantly, CD209 is a receptor of HIV-1 and some other viruses (such as West Nile virus, hepatitis C virus, etc), and some bacteria or parasites. It plays a criti­cal role in capturing and internalizing those pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates transport of HIV to the proteasome.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Extracellular domain of human DC-SIGN\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemistry on frozen tissue sections1 and spatial biology (IBEX)2,3.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Granelli-Piperno A, et al. 2005. J Immunol. 175:4265. (IHC-F) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Gao R, et al. 2021. iScience. 24:103133. PubMed Taffoni C 2022. The EMBO Journal. e111961. PubMed Chometon TQ, et al. 2020. PLoS One. 15:e0231132. PubMed Barrett TJ, et al. 2021. J Thromb Haemost. 19:3139. PubMed Azad A, et al. 2015. J Immunol. 195: 2019-2029. PubMed Svajger U, et al. 2011. J Leukoc Biol. 89:893. PubMed Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed Yu X, et al. 2021. Commun Biol. 4:772. PubMed Shin CH, et al. 2020. J Immunol. 205:3179. PubMed van Doorn CLR, et al. 2021. Front Immunol. 12:712021. PubMed Pahk KJ, et al. 2019. Sci Rep. 9:9050. PubMed\u003cbr\u003e\nRRID: AB_1134060 (BioLegend Cat. No. 330105) AB_1134052 (BioLegend Cat. No. 330106)\u003cbr\u003e\nDistribution: Dendritic cells\u003cbr\u003e\nCell Type: Dendritic cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Granelli-Piperno A, et al. 2005. J Immunol. 175:4265.\u003cbr\u003e\nGene ID: 30835\u003cbr\u003e\nUniProt: View information about CD209 on UniProt.org\u003cbr\u003e\nClone: 9E9A8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862511177897,"sku":"330105","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-330105","provider":"Iright","version":"1.0","type":"link"}